	   SnapGene      zACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCCTCGTTCATTCACGTTTTTGAACCCGTGGAGGACGGGCAGACTCGCGGTGCAAATGTGTTTTACAGCGTGATGGAGCAGATGAAGATGCTCGACACGCTGCAGAACACGCAGCTAGATTAACCCTAGAAAGATAATCATATTGTGACGTACGTTAAAGATAATCATGCGTAAAATTGACGCATGTGTTTTATCGGTCTGTATATCGAGGTTTATTTATTAATTTGAATAGATATTAAGTTTTATTATATTTACACTTACATACTAATAATAAATTCAACAAACAATTTATTTATGTTTATTTATTTATTAAAAAAAAACAAAAACTCAAAATTTCTTCTATAAAGTAACAAAACTTTTATGAGGGACAGCCCCCCCCCAAAGCCCCCAGGGATGTAATTACGTCCCTCCCCCGCTAGGGGGCAGCAGCGAGCCGCCCGGGGCTCCGCTCCGGTCCGGCGCTCCCCCCGCATCCCCGAGCCGGCAGCGTGCGGGGACAGCCCGGGCACGGGGAAGGTGGCACGGGATCGCTTTCCTCTGAACGCTTCTCGCTGCTCTTTGAGCCTGCAGACACCTGGGGGGATACGGGGAAAAGGCCTCCAAGGCCTACTAGTGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGACATATGCTGCAGTCACTTGTACAGCTCATCCATGCCCAGGGTGATGCCAGCGGCGGTCCGAAATTCCAGCAGCACCATGTGGTCCCGCTTCTCGTTGGGGTCCTTGCTCAGCACGCTCTGGGTGCTCAGGTAGTGGCTATCAGGCAGCAGCACGGGGCCATCTCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCCAGCTGCACGCTGCCATCTTCCACGTTGTGCCGGATCTTGAAGTTCACTTTGATGCCGTTTTTCTGCTTCACGGCCATGATGTAGATGTTGTGGCTGTTGAAGTTGTACTCCAGCTTGTGGCCCAGGATGTTGCCGTCCTCTTTGAAGTCCACGCCCTTCAGCTCGATCCGGTTCACGAGGGTGTCGCCCTCGAACTTCACTTCGGCTCTGGTCTTGTAGGTGCCGTCGTCCTTGAAGAAGATGGTCCGTTCCTGCACGTAGCCCTCGGGCATGGCGCTCTTGAAGAAATCGTGCTGCTTCATGTGGTCGGGGTATCTGGCGAAGCACTGCACGCCGTGAGACAGTGTGGTCACGAGGGTAGGCCAAGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCATTTGTGGCGTCGCCTTCGCCCTCTCCCCGCACAGAGAACTTGTGGCCGTTCACGTCGCCATCCAGTTCCACCAGGATGGGCACCACGCCGGTGAACAGTTCCTCGCCCTTGGACACCATGGTGAAGGGTACTGAGCTCGGTACCTGCAGGCGTACCTTCTATAGTGTCACCTAAATGCGATCTGACGGTTCACTTATACTCGAGTTTACTCCCTATCAGTGATAGAGAACGTATGAAGAGTTTACTCCCTATCAGTGATAGAGAACGTATGCAGACTTTACTCCCTATCAGTGATAGAGAACGTATAAGGAGTTTACTCCCTATCAGTGATAGAGAACGTATGACCAGTTTACTCCCTATCAGTGATAGAGAACGTATCTACAGTTTACTCCCTATCAGTGATAGAGAACGTATATCCAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGGTAGGCGTGTACGGTGGGCGCCTATAAAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGCAATTCCACAACACTTTTGTCTTATACTTGGATCCGGGCCCGCGGCCGCCCCGGGACGCGTCATATGGAATTCTACCTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCAGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTAATCTATAACAAGAAAATATATATATAATAAGTTATCACGTAAGTAGAACATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGTAAAAGATAATCATGCGTCATTTTGACTCACGCGGTCGTTATAGTTCAAAATCAGTGACACTTACCGCATTGACAAGCACGCCTCACGGGAGCTCCAAGCGGCGACTGAGATGTCCTAAATGCACAGCGACGGATTCGCGCTATTTAGAAAGAGAGAGCAATATTTCAAGAATGCATGCGTCAATTTTACGCAGACTATCTTTCTAGGGTTAATCTAGCTGCATCAGGATCATATCGTCGGGTCTTTTTTCCGGCTCAGTCATCGCCCAAGCTGGCGCTATCTGGGCATCGGGGAGGAAGAAGCCCGTGCCTTTTCCCGCGAGGTTGAAGCGGCATGGAAAGAGTTTGCCGAGGATGACTGCTGCTGCATTGACGTTGAGCGAAAACGCACGTTTACCATGATGATTCGGGAAGGTGTGGCCATGCACGCCTTTAACGGTGAACTGTTCGTTCAGGCCACCTGGGATACCAGTTCGTCGCGGCTTTTCCGGACACAGTTCCGGATGGTCAGCCCGAAGCGCATCAGCAACCCGAACAATACCGGCGACAGCCGGAACTGCCGTGCCGGTGTGCAGATTAATGACAGCGGTGCGGCGCTGGGATATTACGTCAGCGAGGACGGGTATCCTGGCTGGATGCCGCAGAAATGGACATGGATACCCCGTGAGTTACCCGGCGGGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCAT  2õ                                       '                        !      
                                                            !                              !            #         '                                     	      
   &                               '   =   $                                                                     $                                                                                                                        &                  $                              !   $         (   0                     	         (         !                                                                     ,            #   +                           "                     "                                                                                              ^ Àš›�‘¥Úë./08ÌÍw°¿ÀúæSÕ‰Å §ÁàhœZtÛìù19Î×º
/ûp¦.&TÖÓ®~ˆU $ _ ü €¶Á=xŽÁ�>nù¦ÇDçŠ/ >RôüBmz’¦å±åNÉS
©
ó}i‚ÞÆ] ¨ï8ÏØ°	‚
0üU œÂÜáií:È
ÿ(q Ð'áŒÔ	šÚ·>Vf 7 �uú2úWÅ£
Ý
[º8§Í/Qf×¹’i¯Ê‰Ö .[Üê»
Á@`nÝ ‡uñÒ>�ÂŽµZ]ÕóP
u�#è]¬Â<c?0 ·ý—Í?ú	C
E§¼öLf‹¡è6v % ` ý Â¿ÆÛ„6	Z§]Ä?‹Àÿ �±²MgyÓ¬Í6oùÈÑ’’	‹¶ïÍ' ?SÊÓõýCn“ª·Jë�	G
T
<éö(z~‡Zj ¹`Ü©›g}Ç^ Õ “Ðæô¥æIOÊô#~$1[B Y§�6¦Ô¸Mš-Ðƒ¸{ Ë Y{á@I²×"QC§ª
±
zžHöÅ�ßê …½XÐÙ‹<A
ý6>X[Ÿ/€ ö Ö,�\	€	¤	È	ì	
4
CoqV¼¨Xæ E	i	±	Â-qò[µ&žBn 3 ©·ðè9œË 	d
ƒ
1sQËö£î>›ã3!  ÝâjAî=jÄÇôöf	 rÚÑ�Õ½ÚùrëÛêà? N t Ï BÃØåë–n;YmR÷‚ªk)U[§(âc
aŸ¸Êg
 C í ÐÉ
�CÏZWo (e�ÍV	šfŸØ^	‚	¦	Ê	î	
6
q
¤�ìæ(›  Vž5>DvvA¼(.XÊcÆE9hÎR.ò 8 ø r�Õ3X	|	 	Ä	è	
0
Þ
Ý¨0gØxºpy Š ±­c\k“V°¾¿ qûû.	z
¤
»ju}Ë×@•§Õ×è ½ÑÞ€ 3	¼
XKA;N� K\Ý'.ëÂùajkÞa /�	ð?™<„op`; ò Ò	©:ªj ,Ñ	òÕûe›Ñ4O	4[¥Q
Û
Xx^ÚÀÃ=˜ía cæ3~Wkt?*ÖôV	z	ž	Â	æ	

.
o
Žo¥ôì-9@1 '§ÓÃ�2	J
v£Û kdñ_ y ˆv¡ÖR�õ¶ý^
	$é2˜"­–´«  
˜Î+²Ü@D
¡ó¦7m, ‡ �¸×˜Hyˆê�×ŠÝ½MgŒ
(ã*õ G ¶þb	†	ª	Î	ò	
:
ô
o¾Fd¨÷¢âé wa ƒ)Cû		L
D þ e³Ôtò1:@[UNYIL±Ùçpãl & � ˜ ¤ ¯ ‡£ú
•Äý¨s­Å ©7 Áó ! xÃý)©ÀÝÀÜG	k	³	×	û	
j
[/_œ^"‚@Hî>r) a óÇÅ…Ò«¼Ý7	•™¯È–¤ÿ1·N/Œ¾î ‚Ô‘»„±>
úÄ³#/“
ho 8Ì"7p!	v
Á
Òè‹ÚÒ8“W·fÜP€ , J²­˜
ÉVŒE H�³Nhzam¾Î_HÁ]ðÎ 5 òxØHK¯
÷:!¶!&{Q[ž¥ºª:¾e @TËÔéo”«š,	=VS÷<¡a�>¦œhÈ_ ‹ ¢ F1–Ô‚	H
¿
æ‰ˆ	b €Ø~' f öþ.7D]êì¸ìU
aêÝ)�OkŒšÝAï\åB“! ;S\”—Ñ$T[¦©P`	Š	a
	–XÞ‚˜PS^çî� Ö _¦!˜æ…
"uåðû%L›ÈõD¶5CM  £Ã³ç(?M…´õ$FßÔ;@\ h ’çõ^…¬ßØ#J˜Ë$Q[€$2	#  Z�£P•ÊÜã7ÿã
±æ@C+N™8‰›ð>ö3˜à ø<„_ŒyžÖ
cìø¯Ñ"U ¹0ÿbmKStæ
¹ƒu²ß$„|1' ¸ G¨­º.€‰’{§î	f
3Õ´m}Ž®.Q�œêñ“±¹k†Ås­ Ì ŠÇ+9žÍ÷^¸'W¨Ò²
3ýUÍ¯×àëJ { QŒ½âD³Øæ#Dx´{Ÿ4I÷x…üÓ3O#• |î AJ‡W
~
î
(„âëÅCÆ\ Z«`m¡á8Rs”ß�ä«
µ
àÖ36�y # ] û =Q¦ßûAl™ÀYs�Ë<Œ=mø¥Ô­�ÝT\ ÛîVÄ¯	¹þ7?àm‘ÜhÉÕ= †¾ðÅÑÚ–´B
t\õ  Ê X©?H¤BH	F
�Y¸õ ,~¨0ZšA ¶œ[	h		£	Ç	ë	
3
c
npr,WZš´%  M ì '×äUmÆöž]	�	¥	É	í	
5
�£j€ZÎåÙ`qéßYŸ ÷ =CbqŒÔ-Éby
ÜDwUt½Öo¾-ç	 ñ &-Œ	˜j© x åQVšÐ1	U	y	�	Á	å		
-
n
Úó¿—,•ìð^ª
 Gé áÿ	'+âC& w²Ü¢?»ÜF	j	²	Ö	ú	
ù
T”Ã.®Çrþ¶ÿíX.6=Ÿæoí Ë!lÑÙ"V\O 4 Š èñé0™ÓG9U RÜ ;¤×œ9=ä½&   ‘:–åZ]„¨«`
„
!'>#täÓïú?�—ORÇœ4 · ¸�^”-ÛJzRs	e
2°å˜÷7­�é!…Ä ªÆ_l�ÌàÞÕüÌá5„ûÒÖ <¥ÚÞík˜¨ï>GG÷®	s·ôÿÛg¬ÈÜ™Ô@S[ ë &ãBp‹ÅÈõg	€Ûm+ävips, w v±ÛF¡è>k“ŸÐÙU¾ìþë*áåN©  ‰ �Åçk“ä_
 ="8äÛÒàŽÖúN ¼' v ê %;�¤ÄÙæì—oŠEj=ã^
<ÏÚl*Øß¶ãóþufÇý»¨R= O P u › Ø é CDÙš%&'()*/07M<Z[\]ì]
ƒÎÙVòýGHbcde ÅÆåæ¸¹º§¹Ë78EOPQ$ Q Y ‚ œ § ² Ð Ù ä —#›þ8^n÷Sø(	/8„«ÇM\¨IàçóFh U EÚ+1N�ícW)d'+¡ÍºÌ9 D 4$_ø)	ë
lN‚]ï@è!�Gi  R Z � Ç î Ñøûš$9…�¬²wð¿Ê:[ƒ�Xp ƒ � ¨ œËÿ9{o¯T‡
Ê
0·Ž©ºá ³ Ñ Ú å r˜u�ùvÈà	«ŒDÐJôø( )5ÎºÛ
_	ƒ	§	Ë	ï	
7
XŠLÛœF’» V W,Jž¦©¬Ù�
}¥¯íÄçŽ(: a2O›g ý
d‘ûOÊ¢s÷¡Í Ã FfžgŠîr
ç*e)a,ÎJ÷ W6 ½ËéÇñÆ
&Ï^d�yý 1 ?V
&dd	Ð	¨À:fiðAºéž¥ E Ÿ%¥w¾ùc)/Y<
Ù
mFê¤¯ój 5E”`w6B*	ˆ	ì
äOƒ‡~S"!/cH [ È ï ù Ò	Y	}	¡	Å	é	
1
% –*çV;\š S â sŽùüC‘›¬	[
:{Ž‘­µÞÖÙsqË‹Y  = ‘ y¸Öå
Ä
†ˆŠŒ³x¿1 9 ó4?	�
ß
©ñhÇÀy»z¡„Žq  ) „ {�|°HªfR´  "‡®dÈØâ"|Ž¸3=”W¿â  ? I l “ © ¼ ²ÄÌç:gp„¹{1Aëfz�ä±IÀ ž MT÷ µUˆ
Ë
O]l»�S Ò ™r®ºüú{
»ÁÝ÷EKSu^Ö$   ´ æ Njsv‘¡R	v	š	¾	â	
*
ö
*i˜á
¶¼á�ªÑBÂõ)Ä–ö± ¶éü/	ô	qwÉ�¬ïùëk~Ìù8A‹Ø Û Å2€·
¥
H°ÈH vØRý¨&é ¾ßé÷6® L4	N
-Y‹BÂÎ‡|†d ÏÜ‰:É<&n�w“ *6aÏÒû�…ðÚ¯`	„	¨	Ì	ð	
8
›ÕL]6§G¼ Š»NZ¡‡áq%Ð½
Ô
á
õ&”Y‚MÜOîZ‘¯i ?.]Þ+/šÚø	SÅúÌk�²ßX; W 	-KŸ§ª­õ�
-6~° î¸ÂE) ; j ñ¦bÅ è£˜ %LXë(ì�
ÃÀlÔb b ýJ@=’�{ˆ<	 ^”õNšpPËÖt íP%œ¡h¡;	Ž	’
Ÿ
þ
qø¢   03EA	K	o	“	·	Û	ÿ	#
eñü…²£aùFÎüs ó Gs
ó	¶;0£µk�Ý Ä g"‹`ÁÍîÓ	c«{-ˆÏK# ~ *ï			$	Ð
¢+½Ò4 mŸ'hèjvfª*b«ø ~gµ-Öv�¾ÈR
ò¥-åh. -
7é�
¢
Ç
Ü
'P_e‹™¶ üfÌÒ«Ì
5\òYÐ‘ŠÁç5>µîbuþ> XÒ’óœ5Pê€¦èyL†_Û}ÄŽzé 2  çW+ÿÁBPpñõíZ».AWå2  B d@ÏXl <iÃóe×õe	p
©�¦B¼øên 4u@'W	{	Ÿ	Ã	ç	
/
gj|Ÿ¦ ¼Ÿ2	Ñ	ï;Jƒ:�_:  &}¦jsÔúdô3N3~¤Ú
Ww¢¤ë!³8 F † 	Öµx‡(*B±K
ó
nÜò¥l`ô — ® d(¨¿Ä09Z”›¬£GK°òâk + � 	=
—
^G.ÀDeÛ 6\w•×+	åˆ%a‹�™@îAd ¥ R#S—²Þ×"_	‰	LP„³E:#Kšôµ ¢Fax~‘Éþå
?B®õ#—T0r¬ z ‰7Cåö]·Vwí
2
Tê3®[N"xI \ É ú úŽ³AE
¤+ô§ L ð µalÓ,Z	~	¢	Æ	ê	
2
¢iØÕn$ P™Ï	m
SW-<] Ý&¯—è–8Ã› ˆ °Ú’­	’žh«)ä+Z& T � š ¦ ± ã $Ž–£Ø‰™ý$Dœâë'	\
;ÆÍØL×ÞtßäòüÌ¦ � q3t�Ê÷úzê
u�¶ßª‹î?¾'9Œ Â ¹fI‰¥ü
|®‰úÚN�Érö U½ÿ	c	‡	Ï	;
Å
Ø
¿£xb á ·äB�	>	«	
Z
œ
z‡‰‹�´ÿr   ( > H ’ !†zÃÇ×áæ!f{ƒey2eãH$  M­¹ Q	u	™	½	á	ó	

)
õ
p—ºœÀîG¯©ÇøGRtêÁ7Ãüõ `‰„~5M
Ó
à
“5ÈÁÍY†v{… : >-êðô*÷	±ÓW¢ ôMDü@	J	n	’	¶	Ú	þ	"
ž
Er 		Ï
bªòiuzµ¼‡¢�Ü }f´uè¡
…gæ´tèµ  A ÿ »žÎÿ;hÂòîIOY»{äm * … – ­ Õ|ir†'ó2AM°}£–
ò
 V]«FJ²Ú }±~\Uv1`JZ@MSqw K ´l
h•Â! Ž ™ ¥ ° Á #2¯eˆÉÙ#¤áé
û
tÅÌ©ˆ>ªÞãñ&8¥$   ' à ¬ÂÆàã Te‚�¼=	›
–¹þ®ÆFsÀÂûô ˆ,éï}I	m	‘	µ	Ù	ý	!
’a4t´X†¡Û @ • ¬ | Íhq…
g|•
ñ
\í0_åEIz³?s(  J À ³+«ÁÅßèSd�»àH	l	�	´	Ø	ü	 
k
è
g‘`‡ÝðÁú¤0  	 ” ª « ½ ¾ ¿ yz{Äþÿ*ªÞRºÝÞß”
BCDEì�ÌÍÎ»#$ABCD�²Üï3 
 m Å;Á2F{ Ï¼�%ƒÈá‚J4* Ÿ N�£Š^m–°—Ð K0ÊÂï  Uôø¶ÂÈw†žj¬8	3G|š2ÏO~‚  <?oÆ	Ì
P¡Éâ¸ƒ5T¿+  b n 	ÆVìÓ½Þ‰
Ð½Éž¼qw&„¥� Ó šs¯Õ¤ã	Åøn$F�˜ÑËÃ	 ƒI»}˜C	?
Lð ñr¼ûh
¼ûÂ—±0”–TLi_mp×þ   O‚ý’…‹²â|
_Þ´{‡v1( ! µ OVÃÉ‚”¢xkqS	w	›	¿	ã	
+
w
÷
·×›Óâ9«ÒXü3•*;ÅƒÝ  ç 9t5j}Œ´SnÃö—H�Ò / º õw’·0Â
gâé½ÛŽ”å¸PûQ¯²u! kÍù#‡Ÿ8­"	9	+4H™Óó+¶þ°Cì¥Ð³g÷! ³‹Ç©0	ðìó�BŒ6U, K@ò	õ	Í
Qx¢­„úF  =êýe®ð™
rÊžÊoW¹ÍuÀÙ - p·‘žÊãúl9Ð c ª¯´O¹�Ï¦
Ñ¾Ê±r0¦ÂI!þˆÒ' 
2D3WíGn¸¾
Š
`5½É'^S»ê  {b¿ÔßIIŸx wÙñÇÏ o Ü IÇ‚†¼Æi“M	q	•	¹	Ý	
%
…’ÌcŽ© ¿óy¤Ùäêø7¯äM¨
¹
.ÆùoC|%RÒˆ}‡Ä 6 ›ùZÀÌÿÏ¸Ùe t°±LƒYÔ	5	O
øŒ"\‘¦»ÃKe«;Š¿Ì Ô Éà¶ÏIÖ!¥°
;Ó"·õ'yG™ŸÏ#·f| õ „Õ¼›ÊŠ;D	@
5=Ê¢=ž§â�m s Î AÌê•Q™TÙëb'žÉ U°p¬»~-	?x”Ôñ ÐÝJ>WøMi¨9oi` +bÐ2Õü)I
	n1—Ùj`ÿ Œ‚—Û	a	…	©	Í	ñ	
9
œ½Ö‰c) Œ £ Óòü†sñÑªi
üM^˜
•!�0¨MHqØ½( 7IG€`º½ƒ°u
À
çŠÃ²7Ugn e ¡ -Eë®
¾
ö`µ€NP[¥’ g ^[¢Â—•ZÞWÝ]æCíL, ÷ÿ/;ƒO‹¬¹þlˆâíÕ
â
bë*‚³lt|ˆ�P›±Þï=ð2’°jß *8P‹¼í r“&†Œ³ÑãV
}
´
'ßƒ2ÄBw 2” " P@W¿rÄÊ;•£UlÃ	¸œÓßl}�Y< <T%a›‹	b
x
—YÍY™³T_½èÞž# ÊäU 0ºÛT	x	œ	À	Õ	ä	ù	

,
ø
Ø­Æ!ýàþ–+”
5ïì+ ¶ Ž/]^•˜Òß,FY\ƒ§ªÚIyQr
TÔãû:6¬ù4€ƒQ<ÆÑÕ„% u§F çF!7~ãTMo˜MÓ X × è :".6Liö.7bµñüF&*Ä·6DN  Æ 
™®†
#k±v�ï¹÷ œÉ‚ 4`¨«Ž
æÃK`öE‘I 0 “¤5b%èð§ã†%e}c�é¹¤®ü  < xòÃ
¾•ÆÕ)æU™  k » LSö�´¸z@NêG<Q€R³ iÄ1µè¶)hµÜöàAQŠ]%°" ÎúMYèö†­àïpˆÙ$9K®ÏšÔô%,\�í%m¦®hc i $™Œ
,5RÄ·¿ÁÿËD— ]“ì$ :	‘
I€±z‡Õø
û } l!&)_ÀÌí#	¡/Ñ3´" ‘,ŒËª€
çñ¤,KOäŠ|‰4�=- þ1	A‚9÷Ž^V
 c´8“š¡-¢¿ñC7á" [¤Q"–ÈÝäÖ !	ä
K²>AD9Šœ­?íô™ó ù`k�OR£®×Ô# € p•¢ö&	KíùÈû  L…AzŸ	P	t	˜	¼	à	
(
Y
×
yd›íqQ6G _=Lóö	Î
Ò
°ÌÒ…uV º&}þL¯ÁñTu¢ç 
ÿ
„Xºv ß 1î<	š
sË³Er%…Ú  Q c€	f�	“
ç
ð
ŸË†ºÇày€Ûù2£  €>nëv�¢i‰	pvÏÎ}ÉÁ ðHq|—âá	B	g
z†�•lý . ¹ 4/�“fò*Öµý~¯³Räëûm”¤:²úG®Ñt  ~ñŠd¨ï�n�òtÏ 1…©®»C¸F’L	p	”	¸	Ü	 
$
4/‘ŸËbº‡ÆÑ È£°µÎãø‚XÓ§
¸
Ò¿ËôþxÎ·Jd"¶Ø‰{ r Í ô ¯ÉPº4Øêh§8áÓl H‹_¹Ð(©t
m
/f( d ),:ŠŸ«Ák–­
³
V²s1°Ã<1¤ÿì‘ÞK �¾Éãq:EXÙHT¹ÂÔ	
¬ 5ø|ý“¼ÐÔ	4ë$ Å 3hEEa6âJ_�L hKRŒ4a´ç$yµïd|(T‰P­$ | \ #îo¿Î‹
 ¾Êy†.­–ú !+Õ
	
£J ã,8‰¡¬¾]ò  o¡jK%	O	s	—	»	ß	
'
X
Ñ
JìÇÓ5  Þ |uÀˆæ	ï
…ÆÎDxÚÈ }ï„Bðc–á�à)y”Ð q ®(‰¢ÀÈâj•¬
·
×élwg.7cez [JÈgp9Sá’¬�¬ù Ý n iƒt•àŽåN	r	–	º	Þ	
&
7ˆ“«Í  p ¬­abcdefno‡ˆ½¾¿Ç”¶
k456†‡+,-d‘ª«  +2  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
   	                                                                                                                                 !                                                                                                        	                                                                                                                1                                       	                                                   
                                                         W   N                  !   S   	                                                                                                                                                                                                                                               
                                                                                                                                                                                                                                                                                    
 	                                                 !                                      	                                        1             	                 
                   W N      ! S 	  g!  gó  h  h%  k…  o¡  tÃ/.?NH]_ï$ô :‡ˆ‰ŠÇâã4HEJø
°Ç`ˆÏí(ƒ¸iõ:QA×÷è*-UËé.0QoGSºØA€‰JWì{Öýâb¥S×¿| 0`ªì	0`0`ˆÀÏí#	]üc(Ø›æ@, f¯
Qû·A	
	Ñá€’m±Lx
.	w
ÒrËp9xà �?H¾Ê\A			C‡µÊ	T
è1«²3 ð£F»àÖ!¦°
yGéœ § þ8nSÉ
/÷vi�T
}½
èN®� 	d
1"« " ¢ÈââB	·
©
È=åÉ©
" y£
©¦ 	d
©
1ˆ9•ýT
…F7 2Ý
§f¹È’=Q"		ö	ñ°Võý·ëT
é(jÜ` ‡œ¨§gx°µÊ	T
£
©
è-1�¸‹€
FºXRmÑpÔ¨`ÙšÔ¾pq¢£â+E9:SáEæï­
¶
·
4£ãˆ�³M¯�¾Ê¶Iˆ›QÇÍWÒóµê°
+R^ÝÖ!p°
ø‘9Ôi‚ï©¦å�É 	d
©
1"« (/ ¸èEEæï ~nf	Çò xŒ�êšÍÔ D…Øß#St	«
$…æ€{4¡÷LÓï­
£ú
£…èú£
itÊÖÖçTÜTîI
¢â·
­
àÝð¤0Âþ 	d
1€
÷Fviº•Y§€QÈ>AôÉ
ƒÃ
£ú
,c£m…«Ö7	G	k	³	×	û	
J
o
ú
_Ž"
 / Íý‡ð8}·n¾Ê$ÑW¹n¸»®½
2¦^ÇÍŽFÚ­
6<1Þ3àB	Q"	ŽÚ6B	RXmÑ � w}ò j¯�Ë�.(BCÂ ‰œþúú£
ºiÊÖ¤
u×§è¾q+:TÂ
£Jã|‰, ,¨Z£P\”—Ñ•[¦©ÜãÿPã
	±@C8‰›>‚P˜Yz²Mgy±QÍ©
ª
�ÞïÍ¢£âã§
·
¸
wcz>Õó�?0k"	Ð³!²MgyÍïÍ\”—Ñ[¦©P	‚PZ£P•Üãÿã
±@C8‰›>˜h´çµ‰$V5¼ÊÆÎß ÎŠ^¸'¨+9Z£P\”—žÑ•H[¦©ÜãÿP¯
²
ã
	:±!@C¶&U8‰›ž¯>‚P˜ëeJ¾q¢£âã+:TÂ
§
·
¸
£Jwã|‰czÃ(´õß@\DvAR.{ˆ<Éã¹Ô	
¬ ý“	4ë�uWÅ8ÍQÄ1¶Q%�“Öû”:þAVZÛº
H�hGb/Ú4	ø	N
s
‹’ÅÂ	ß�“þAÖû”:VäU0T	x	œ	À	ä	
,
–+”ïI€z‡
 ¼
N�F‚¿
æ‰~(‰Àj•¬
òxØ{Q/ð\kC„Ï[`ÐÝºƒ°WÃ²MgnšO¹0	w
X¦�ÒÝUuˆ	ÎÈÝjîörÚÛ?¸Ì~Hˆ"7!	ÒsMŒ f 9õâAÄÇôf	 r =êoÍÀ[/ð„`Ì~"7!	Òf9\kCÏ+9žH¯
²
:!¶&Už¯ëeJäU0T	x	œ	À	ä	
,
I€z‡–+”ï
?dd	¨Aéô¶O~YdÏ'e ¡ -Eë®
¾
ö`µ€NP[¥’d ),:ŠŸ«Ák–­
³
V²s1°Ã<1¤ÿì‘ÞK5 òxØHK¯
÷:!¶!&{Q[ž¥ºª:¾eƒ)Cû		L
D· ¸�^”-ÛJzRs	e
2°å˜÷7­�é!…Äf öþ.7D]êì¸ìU
aêÝ)�OkŒšÝAï\åB“ù`k�OR£®×Ô#qûû.	z
¤
»ju}Ë×@•§Õ×èË Y{á@I²×"QC§ª
±
zžHöÅ�ßêÈ£°µÎãø‚XÓ§
¸
Ò¿ËôþxÎ·Jd"¶Ø‰{0 “¤5b%èð§ã†%e}c�é¹¤®ü · Ë Yª¸ÆY{�á^_l�§Ìà@I”-�²×Û"6JQz¦ÞCRs§	e
ª
±
2°Ôåzž¸ÕüM˜Ìá÷7Hš­ö-�é5„ÅÐûƒ�¸ÒÖß!{…Äê0 < á x“¤·äòBU�½ÿ	5b%èð	>	c	‡	«	Ï	
;
Z
œ
Ã
Å
Ø
z‡‰‹�§´¿ãÿ†¾%e}c�•ÆÕé)ræ£¹Ux¤®ü b™v*6aÏÒû�…ðÚ¯`	„	¨	Ì	ð	
8
›ÕL]6§G¼¥ R#S—²Þ×"_	‰	LP„³E:#Kšôµƒ � ¨ œËÿ9{o¯T‡
Ê
0·Ž©ºá}ï„Bðc–á�à)y”Ð…½XÐÙ‹<A
ý6>X[Ÿ/€Ž ™ ¥ ° Á #2¯eˆÉÙ#¤áé
û
tÅÌ©ˆ>ªÞãñ&8¥~ · *6a†¸Ï�Òû^�…¾ð*”ÅÑÚï-–ÛðJz´ÚRs¯				$	`	„	¨	Ì	ð	
8
B
e
Ð
›Õ2°åL]t¢6˜÷+7­\�½éõ §Ò4!G…Ä¼ƒI»}˜C	?
Lð  4Ë     ¶  �         �{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’6ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž�¼Ì<ÿÌ¶`Zçç9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰º[™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,"<?xml version="1.0"?><Features nextValidID="27"><Feature recentID="26" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2224-2226" color="#a6acb3" type="standard"/></Feature><Feature recentID="25" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2227-2239" color="#ffcc99" type="standard"/></Feature><Feature recentID="24" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="23" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2205-2223" color="#ff0000" type="standard"/></Feature><Feature recentID="22" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="21" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="20" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="19" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="18" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3132-3720" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4682" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="3891-4682" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="4683-4751" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="2778-2784" color="#ffffff" type="standard"/><Segment range="2785-2802" color="#ffffff" type="standard"/><Segment name="-35" range="2803-2808" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2823-2844" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2730-2746" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2754-2770" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1812-1933" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1375-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1728-1759" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>1772863c-6a01-4be8-ac68-e566cf24ddda</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="7:3:35">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..4750" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..4750" colors="black"/></BottomStrand></StrandColors>  ^X     
#  'c        'c{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’6ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž�½‡Ë¥ÑÕAUCHªgQ‡ÕD+«Q3„æ¥[Ãšö¶©Ð¾<0CUû©3:9US/‰c†7mÄíuÕ–ƒ§�ÄÕT–ÇÒr²¸¹•Tat!!WƒAƒÆŽ…8•Vg8jqÅ©ì®SãYfêæÆiPÈãÎ&•X|oª#26üjó-ºÝMoNcÃ&aœÔc:ìt)iö*hi•±ÑÓ¥ÁprEaLÍÖ‰s²M\ŒWÎàGtÓ¤øÀë:a³Ÿè÷4Ôv¾l`RQªÕÊblf¨Ûo:}X¨QV5TÕ<U[j‘E‰E™áEQèUã]Z¤º¥MrÄ�ü†ÕÅt&¦j¤ÏkWTqE¨3È‘AuS…vªÎuG0æ	4áIñ…Z›o–ÏU©¥�µ\ÒM1¦Qqœ)¯[N &µmTQÉT®¨~¦óJ…SŠ½1µAU¡K
•IhU„URWo…èEI´ÛÉTmÓÂša2k^�ƒµQŠž¿�UµŠ®ó¡E4ÁM5SV×Æ:¥s.®–—ÄQ…1J†RVÍ…^GTñªÚtÂúŽ™jˆ]¬ùT‘DTßƒ°^»ií]Or„oCøŽ7S²?ö×à²l1#AD<u4âqøýöôÑNÓ�b±8FÿSëÐUD^8aÄlõ0ÌF„ÒEáýÁaMsDV…Íuj’ê•44ËUþòZxš™Â¥¯]TSM=‘<«Û¦P
‡]­âZ2¡´!Ã‰*å8áßšÑU!­EU�4„àãAU6­¶ÜW	<ê'Zœm¯iV¨ßjeBT¸ÐÓš¥ÕFDZÞã­AXQ X…Zž!†tN•TÓÅ…L…LU]Ei-é·ÐQÒª…QUYNQi­Õ]R"FvNU›:˜{ŠUE MQÉx3NF�tÈ«®ü»�yJÉ\VE­5C¡VT8¨RØ<þm«±:ŒÈëui#^»ií]Or„oCø„øSDØelþ¾Ç!¾Pm@Çá¶îlðÈ‚'àn#†ïL�_à�T@×ÝOÛ}14¬u4TA8`sßOÓRVû€Óß‚L3ÜZ«(¡MN b…@ø�?"ý\4[ß�¿CÅÐÓ²|†ÄãŒ4l�pÜöD�CþMnlþ_�DQ�ÄáQ¬<Aø]±øîOŽe4×V©.©SCA±_à òÝv4M7Û r8Fø@Ë!³À·H³Ç ^8}µÃ†È=2PõEl6‚ùøM5=uÔ;�Duãq0áWxEÕ3™Ïh3^»ií]Or„oCø„ßôëMõ]þž²=5ÔÜ:“ìvÓár òL7Øm3ÕzÇŸ„Äv±D1H?Cø¸M*Oƒ“ûÄ¹`Å<C÷­½øQwÔnxÃ5ôÔFëƒ0Ôlñëà[LsÔ½ôÔRôí­\{÷ªÊ(SS€¡D_Ð>!‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²~‡ç�#¬üçü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×Ou™·ÏM\6Ô!zÔ=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô”Zçç9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰º[™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6  ¿  
i  
¦  ¢  ó    A  y  5  F  ¬  ½  •  ¦   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Oâ<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1375-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7144-7265" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8086-8102" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8062-8078" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8155-8176" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8110-8116" color="#ffffff" type="standard"/><Segment range="8117-8134" color="#ffffff" type="standard"/><Segment name="-35" range="8135-8140" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10014" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9223-10014" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10015-10083" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8464-9052" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6365" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="6312-6368" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5876" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="5823-5879" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5501" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="5448-5504" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5144" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="5091-5147" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4784" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="4731-4787" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3899" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="3846-3902" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3560" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="3507-3563" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2720" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091" detectionMode="exactProteinMatch"><Segment range="2667-2723" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="GvpG" directionality="1" translationMW="10440.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="3567-3827" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="GvpS" directionality="1" translationMW="10290.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="4791-5072" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="GvpK" directionality="1" translationMW="10316.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="5151-5429" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="GvpJ" directionality="1" translationMW="11129.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="5508-5804" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GvpU" directionality="1" translationMW="14827.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="5883-6293" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="GvpN" directionality="1" translationMW="34696.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="1728-2651" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="GvpF" directionality="1" translationMW="28861.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="2727-3491" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="GvpL" directionality="1" translationMW="31145.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7091" maxFusedRunOn="7091"><Segment range="3906-4712" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="EmGFP" directionality="1" translationMW="26897.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6372-6374" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6375-6377" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6378-7091" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Emerald GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7537-7555" color="#ff0000" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7559-7571" color="#ffcc99" type="standard"/></Feature><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7556-7558" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>a596e1e3-5f8f-4867-a0ce-d4b5ee014535</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="7:2:36">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..6370" colors="black"/><ColorRange range="7091..10082" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6370" colors="black"/><ColorRange range="7091..10082" colors="black"/></BottomStrand></StrandColors>  ^X     
"  '_        '_{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž�½‡Ë¥ÑÕAUCHªgQ‡ÕD+«Q3„æ¥[Ãšö¶©Ð¾<0CUû©3:9US/‰c†7mÄíuÕ–ƒ§�ÄÕT–ÇÒr²¸¹•Tat!!WƒAƒÆŽ…8•Vg8jqÅ©ì®SãYfêæÆiPÈãÎ&•X|oª#26üjó-ºÝMoNcÃ&aœÔc:ìt)iö*hi•±ÑÓ¥ÁprEaLÍÖ‰s²M\ŒWÎàGtÓ¤øÀë:a³Ÿè÷4Ôv¾l`RQªÕÊblf¨Ûo:}X¨QV5TÕ<U[j‘E‰E™áEQèUã]Z¤º¥MrÄ�ü†ÕÅt&¦j¤ÏkWTqE¨3È‘AuS…vªÎuG0æ	4áIñ…Z›o–ÏU©¥�µ\ÒM1¦Qqœ)¯[N &µmTQÉT®¨~¦óJ…SŠ½1µAU¡K
•IhU„URWo…èEI´ÛÉTmÓÂša2k^�ƒµQŠž¿�UµŠ®ó¡E4ÁM5SV×Æ:¥s.®–—ÄQ…1J†RVÍ…^GTñªÚtÂúŽ™jˆ]¬ùT‘DTßƒ°^»ií]Or„oCøŽ7S²?ö×à²l1#AD<u4âqøýöôÑNÓ�b±8FÿSëÐUD^8aÄlõ0ÌF„ÒEáýÁaMsDV…Íuj’ê•44ËUþòZxš™Â¥¯]TSM=‘<«Û¦P
‡]­âZ2¡´!Ã‰*å8áßšÑU!­EU�4„àãAU6­¶ÜW	<ê'Zœm¯iV¨ßjeBT¸ÐÓš¥ÕFDZÞã­AXQ X…Zž!†tN•TÓÅ…L…LU]Ei-é·ÐQÒª…QUYNQi­Õ]R"FvNU›:˜{ŠUE MQÉx3NF�tÈ«®ü»�yJÉ\VE­5C¡VT8¨RØ<þm«±:ŒÈëui#^»ií]Or„oCø„øSDØelþ¾Ç!¾Pm@Çá¶îlðÈ‚'àn#†ïL�_à�T@×ÝOÛ}14¬u4TA8`sßOÓRVû€Óß‚L3ÜZ«(¡MN b…@ø�?"ý\4[ß�¿CÅÐÓ²|†ÄãŒ4l�pÜöD�CþMnlþ_�DQ�ÄáQ¬<Aø]±øîOŽe4×V©.©SCA±_à òÝv4M7Û r8Fø@Ë!³À·H³Ç ^8}µÃ†È=2PõEl6‚ùøM5=uÔ;�Duãq0áWxEÕ3™Ïh3^»ií]Or„oCø„ßôëMõ]þž²=5ÔÜ:“ìvÓár òL7Øm3ÕzÇŸ„Äv±D1H?Cø¸M*Oƒ“ûÄ¹`Å<C÷­½øQwÔnxÃ5ôÔFëƒ0Ôlñëà[LsÔ½ôÔRôí­\{÷ªÊ(SS€¡D_Ð>!‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²~‡ç�#¬üçü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×Ou™·ÏM\6Ô!zÔ=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô”Zçç9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰º[™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6  »  
e  
¢  ž  ï     =  u  1  B  ¨  ¹  ‘  ¢   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Oâ<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1376-1394" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1412-1430" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1448-1466" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1484-1502" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1520-1538" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1556-1574" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1592-1610" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1371-1717" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7140-7261" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8082-8098" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8058-8074" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8151-8172" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8106-8112" color="#ffffff" type="standard"/><Segment range="8113-8130" color="#ffffff" type="standard"/><Segment name="-35" range="8131-8136" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10010" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9219-10010" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10011-10079" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8460-9048" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6361" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="6308-6364" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5872" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="5819-5875" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5497" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="5444-5500" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5140" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="5087-5143" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4780" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="4727-4783" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3895" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="3842-3898" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3556" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="3503-3559" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2716" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087" detectionMode="exactProteinMatch"><Segment range="2663-2719" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="GvpG" directionality="1" translationMW="10440.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="3563-3823" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="GvpS" directionality="1" translationMW="10290.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="4787-5068" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="GvpK" directionality="1" translationMW="10316.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="5147-5425" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="GvpJ" directionality="1" translationMW="11129.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="5504-5800" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GvpU" directionality="1" translationMW="14827.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="5879-6289" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="GvpN" directionality="1" translationMW="34696.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="1724-2647" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="GvpF" directionality="1" translationMW="28861.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="2723-3487" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="GvpL" directionality="1" translationMW="31145.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7087" maxFusedRunOn="7087"><Segment range="3902-4708" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="EmGFP" directionality="1" translationMW="26897.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6368-6370" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6371-6373" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6374-7087" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Emerald GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7533-7551" color="#ff0000" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7555-7567" color="#ffcc99" type="standard"/></Feature><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7552-7554" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>a596e1e3-5f8f-4867-a0ce-d4b5ee014535</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:40:54">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..6366" colors="black"/><ColorRange range="7087..10078" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6366" colors="black"/><ColorRange range="7087..10078" colors="black"/></BottomStrand></StrandColors>  ^W     
!  'Y        'Y{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èØ|º]TT4�:¦uqTBºµ8NjU¼9¯kj�ãÃ5_º“13£•Q2ø–8cpÜN×]Q©h1ªpÜMUIl}'++‹™UFBx4<hèS‰U`æs†§ZžÊå>1Å–n®lf‘EŽ<â`IU‡Æú¢3#oÆ¯2Û­ÔÖôá&<2fÍF3®ÇB–Ÿb¦†™QÛ1ª\W$QFÌÝh—;$ÕÈÅ|îqWM0zOŒ³¦9þ�sMGkæÆ!Å­\¦&Æj�¶ó§ÕŠ�…cUMSÅU¶©X”Y‘ÞUN…^5ÕªKªTÐÐG,AWøÈa!]\WBjfªLöµpeGZƒ<�IU8Wj¡¬çTs`“NŸU©¶ùlõZšXÛUÍ$Ó`uÂšõ´êbkVÕE•Jê�Wêo4¨U8«ÓTZ°©T–€UQ`èEU%qVø^„T›M¼•AVÑM<)¦&µèX;U©ëøÕQKQÑˆªï:SLÓU5a=p1\cªW2êéi|ES¨aU%aLØUàTuO­§L/ øé–¨…ÚÏ•IEMø;1õë¶žÕÔðG(Aô?ˆãu;!óÿm~!FÃB4CÇSN'�ßoMá½8Ñ†+€Äoñ>½TAÅã†œFÏSÄhM ^ÑLT×4Eh\×V©.©SCA±_à!€E§‰©œ*ZõÕE4ÓÙÊ½ºe ¨pEÚÞ'õ£*B8’®S�Žù­RÔUXA˜N4SjÛmÅp“Î¢qE©ÆÚö•j�ö¦T!EK�9¡]QTdE­áÎ:Ô…ˆU©âgDéUAm1\XTÈTÅUÔV’Þ›}*¨UU‘´á¡U‘:ÝUÕ"!DgaÔåQi³©‡¸¥TZÕ—ƒ4át`ÈLŠºïË¸Ñ§”¬•ÅdQ:ÓT:eC�J…!À}ƒÏæÚ»¨ÌŽ·V’1õë¶žÕÔðG(Aô?ˆO…4M�PfÏá±Ûìràu öÔ~næÏ�ø"~á8nôÈþÐõD}Ôý·ÓJÇSED†=ðôý5 uo¸0Mø$Ã=Åª²ŠÔà(QôˆÓò/ÐõÃE½øñ´<]àý;!‡ÈlN8ÃFÈÏaH0ßáñÄÑ±àÏá…øENŠÁàsÄ…Û€þà4øáÆQƒAuj’ê•44ËUþò ]×cDÓ}²#�To�Ô²<Ût€[<rã‡ÛQ¼8lƒÓ uTVÃ`h qÿŸ„ÓP3×]C¸AWQŽ7w�DQ±S9‘Löƒ1õë¶žÕÔðG(Aô?ˆMðÿN´ßPußàYë!CÓ]MÃ©>Çm>!`!¤Ã}†Ó=Q'¬yøLGk@Óƒô?‹„Ò¤ø91¼K–SÄ?zÛß…}FçŒ1ÓQ7ÿM@Ôn¸3FÏ�Á®¾U´Ç=KßME/NÚÕÇ±Ïqj¬¢…58ŠEýâ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷è~pÈÒ:ÏÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷P¹›|ñÔÑEÃmB­GóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IE®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¥¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  µ  
_  
œ  ˜  é  ú  7  o  +  <  ¢  ³  ‹  œ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Oâ<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7134-7255" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8076-8092" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8052-8068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8145-8166" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8100-8106" color="#ffffff" type="standard"/><Segment range="8107-8124" color="#ffffff" type="standard"/><Segment name="-35" range="8125-8130" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10004" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9213-10004" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10005-10073" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8454-9042" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6355" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="6302-6358" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5866" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5813-5869" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5491" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5438-5494" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5134" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5081-5137" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4774" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="4721-4777" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3889" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3836-3892" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3550" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3497-3553" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2710" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="2657-2713" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="GvpG" directionality="1" translationMW="10440.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3557-3817" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="GvpS" directionality="1" translationMW="10290.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="4781-5062" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="GvpK" directionality="1" translationMW="10316.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5141-5419" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="GvpJ" directionality="1" translationMW="11129.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5498-5794" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GvpU" directionality="1" translationMW="14827.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5873-6283" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="GvpN" directionality="1" translationMW="34696.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="1718-2641" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="GvpF" directionality="1" translationMW="28861.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="2717-3481" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="GvpL" directionality="1" translationMW="31145.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3896-4702" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="EmGFP" directionality="1" translationMW="26897.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6362-6364" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6365-6367" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6368-7081" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Emerald GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7527-7545" color="#ff0000" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7549-7561" color="#ffcc99" type="standard"/></Feature><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7546-7548" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>a596e1e3-5f8f-4867-a0ce-d4b5ee014535</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:39:52">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></BottomStrand></StrandColors>  _9     
!  'Y        'Y{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èØ|º]TT4�:¦uqTBºµ8NjU¼9¯kj�ãÃ5_º“13£•Q2ø–8cpÜN×]Q©h1ªpÜMUIl}'++‹™UFBx4<hèS‰U`æs†§ZžÊå>1Å–n®lf‘EŽ<â`IU‡Æú¢3#oÆ¯2Û­ÔÖôá&<2fÍF3®ÇB–Ÿb¦†™QÛ1ª\W$QFÌÝh—;$ÕÈÅ|îqWM0zOŒ³¦9þ�sMGkæÆ!Å­\¦&Æj�¶ó§ÕŠ�…cUMSÅU¶©X”Y‘ÞUN…^5ÕªKªTÐÐG,AWøÈa!]\WBjfªLöµpeGZƒ<�IU8Wj¡¬çTs`“NŸU©¶ùlõZšXÛUÍ$Ó`uÂšõ´êbkVÕE•Jê�Wêo4¨U8«ÓTZ°©T–€UQ`èEU%qVø^„T›M¼•AVÑM<)¦&µèX;U©ëøÕQKQÑˆªï:SLÓU5a=p1\cªW2êéi|ES¨aU%aLØUàTuO­§L/ øé–¨…ÚÏ•IEMø;1õë¶žÕÔðG(Aô?ˆãu;!óÿm~!FÃB4CÇSN'�ßoMá½8Ñ†+€Äoñ>½TAÅã†œFÏSÄhM ^ÑLT×4Eh\×V©.©SCA±_à!€E§‰©œ*ZõÕE4ÓÙÊ½ºe ¨pEÚÞ'õ£*B8’®S�Žù­RÔUXA˜N4SjÛmÅp“Î¢qE©ÆÚö•j�ö¦T!EK�9¡]QTdE­áÎ:Ô…ˆU©âgDéUAm1\XTÈTÅUÔV’Þ›}*¨UU‘´á¡U‘:ÝUÕ"!DgaÔåQi³©‡¸¥TZÕ—ƒ4át`ÈLŠºïË¸Ñ§”¬•ÅdQ:ÓT:eC�J…!À}ƒÏæÚ»¨ÌŽ·V’1õë¶žÕÔðG(Aô?ˆO…4M�PfÏá±Ûìràu öÔ~næÏ�ø"~á8nôÈþÐõD}Ôý·ÓJÇSED†=ðôý5 uo¸0Mø$Ã=Åª²ŠÔà(QôˆÓò/ÐõÃE½øñ´<]àý;!‡ÈlN8ÃFÈÏaH0ßáñÄÑ±àÏá…øENŠÁàsÄ…Û€þà4øáÆQƒAuj’ê•44ËUþò ]×cDÓ}²#�To�Ô²<Ût€[<rã‡ÛQ¼8lƒÓ uTVÃ`h qÿŸ„ÓP3×]C¸AWQŽ7w�DQ±S9‘Löƒ1õë¶žÕÔðG(Aô?ˆMðÿN´ßPußàYë!CÓ]MÃ©>Çm>!`!¤Ã}†Ó=Q'¬yøLGk@Óƒô?‹„Ò¤ø91¼K–SÄ?zÛß…}FçŒ1ÓQ7ÿM@Ôn¸3FÏ�Á®¾U´Ç=KßME/NÚÕÇ±Ïqj¬¢…58ŠEýâ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷è~pÈÒ:ÏÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷P¹›|ñÔÑEÃmB­GóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IE®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¥¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  µ  
_  
œ  ˜  é  ú  7  o  +  <  ¢  ³  ‹  œ   ˆ       ®ZTR

BASE       i  xÁA  ƒ°Yãx` þµ,9ImM¥,	¦èŠ©1)¤Â•JÎm1#—Ë’˜­ÀSA´‚²D]hIÌæ*˜©‹º‚b…‘ˆZ›…+L£Ð2CW©s•¤ËµxªˆMk   ƒ<AlignableSequences trimStringency="Medium"><Sequence name="GVPK" ID="0" sortOrder="1" trimmedRange="0..281"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Oâ<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7134-7255" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8076-8092" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8052-8068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8145-8166" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8100-8106" color="#ffffff" type="standard"/><Segment range="8107-8124" color="#ffffff" type="standard"/><Segment name="-35" range="8125-8130" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10004" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9213-10004" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10005-10073" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8454-9042" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6355" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="6302-6358" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5866" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5813-5869" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5491" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5438-5494" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5134" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5081-5137" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4774" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="4721-4777" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3889" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3836-3892" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3550" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3497-3553" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2710" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="2657-2713" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="GvpG" directionality="1" translationMW="10440.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3557-3817" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="GvpS" directionality="1" translationMW="10290.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="4781-5062" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="GvpK" directionality="1" translationMW="10316.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5141-5419" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="GvpJ" directionality="1" translationMW="11129.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5498-5794" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GvpU" directionality="1" translationMW="14827.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5873-6283" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="GvpN" directionality="1" translationMW="34696.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="1718-2641" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="GvpF" directionality="1" translationMW="28861.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="2717-3481" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="GvpL" directionality="1" translationMW="31145.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3896-4702" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="EmGFP" directionality="1" translationMW="26897.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6362-6364" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6365-6367" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6368-7081" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Emerald GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7527-7545" color="#ff0000" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7549-7561" color="#ffcc99" type="standard"/></Feature><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7546-7548" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>a596e1e3-5f8f-4867-a0ce-d4b5ee014535</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:39:0">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></BottomStrand></StrandColors>  _:     
!  'Y        'Y{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èØ|º]TT4�:¦uqTBºµ8NjU¼9¯kj�ãÃ5_º“13£•Q2ø–8cpÜN×]Q©h1ªpÜMUIl}'++‹™UFBx4<hèS‰U`æs†§ZžÊå>1Å–n®lf‘EŽ<â`IU‡Æú¢3#oÆ¯2Û­ÔÖôá&<2fÍF3®ÇB–Ÿb¦†™QÛ1ª\W$QFÌÝh—;$ÕÈÅ|îqWM0zOŒ³¦9þ�sMGkæÆ!Å­\¦&Æj�¶ó§ÕŠ�…cUMSÅU¶©X”Y‘ÞUN…^5ÕªKªTÐÐG,AWøÈa!]\WBjfªLöµpeGZƒ<�IU8Wj¡¬çTs`“NŸU©¶ùlõZšXÛUÍ$Ó`uÂšõ´êbkVÕE•Jê�Wêo4¨U8«ÓTZ°©T–€UQ`èEU%qVø^„T›M¼•AVÑM<)¦&µèX;U©ëøÕQKQÑˆªï:SLÓU5a=p1\cªW2êéi|ES¨aU%aLØUàTuO­§L/ øé–¨…ÚÏ•IEMø;1õë¶žÕÔðG(Aô?ˆãu;!óÿm~!FÃB4CÇSN'�ßoMá½8Ñ†+€Äoñ>½TAÅã†œFÏSÄhM ^ÑLT×4Eh\×V©.©SCA±_à!€E§‰©œ*ZõÕE4ÓÙÊ½ºe ¨pEÚÞ'õ£*B8’®S�Žù­RÔUXA˜N4SjÛmÅp“Î¢qE©ÆÚö•j�ö¦T!EK�9¡]QTdE­áÎ:Ô…ˆU©âgDéUAm1\XTÈTÅUÔV’Þ›}*¨UU‘´á¡U‘:ÝUÕ"!DgaÔåQi³©‡¸¥TZÕ—ƒ4át`ÈLŠºïË¸Ñ§”¬•ÅdQ:ÓT:eC�J…!À}ƒÏæÚ»¨ÌŽ·V’1õë¶žÕÔðG(Aô?ˆO…4M�PfÏá±Ûìràu öÔ~næÏ�ø"~á8nôÈþÐõD}Ôý·ÓJÇSED†=ðôý5 uo¸0Mø$Ã=Åª²ŠÔà(QôˆÓò/ÐõÃE½øñ´<]àý;!‡ÈlN8ÃFÈÏaH0ßáñÄÑ±àÏá…øENŠÁàs„…Û€þà4øáÆQƒAuj’ê•44ËUþò ]×cDÓ}²#�To�Ô²<Ût€[<rã‡ÛQ¼8lƒÓ uTVÃ`h qÿŸ„ÓP3×]C¸AWQŽ7w�DQ±S9‘Löƒ1õë¶žÕÔðG(Aô?ˆMðÿN´ßPußàYë!CÓ]MÃ©>Çm>!`!¤Ã}†Ó=Q'¬yøLGk@Óƒô?‹„Ò¤ø91¼K–SÄ?zÛß…}FçŒ1ÓQ7ÿM@Ôn¸3FÏ�Á®¾U´Ç=KßME/NÚÕÇ±Ïqj¬¢…58ŠEýâ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷è~pÈÒ:ÏÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷P¹›|ñÔÑEÃmB­GóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IE®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¥¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  µ  
_  
œ  ˜  é  ú  7  o  +  <  ¢  ³  ‹  œ   ˆ       ®ZTR

BASE       i  xÁA  ƒ°Yãx` þµ,9ImM¥,	¦èŠ©1)¤Â•JÎm1#—Ë’˜­ÀSA´‚²D]hIÌæ*˜©‹º‚b…‘ˆZ›…+L£Ð2CW©s•¤ËµxªˆMk   ƒ<AlignableSequences trimStringency="Medium"><Sequence name="GVPK" ID="0" sortOrder="1" trimmedRange="0..281"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Oâ<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7134-7255" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8076-8092" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8052-8068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8145-8166" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8100-8106" color="#ffffff" type="standard"/><Segment range="8107-8124" color="#ffffff" type="standard"/><Segment name="-35" range="8125-8130" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10004" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9213-10004" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10005-10073" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8454-9042" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6355" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="6302-6358" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5866" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5813-5869" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5491" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5438-5494" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5134" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5081-5137" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4774" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="4721-4777" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3889" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3836-3892" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3550" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3497-3553" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2710" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="2657-2713" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="GvpG" directionality="1" translationMW="10440.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3557-3817" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="GvpS" directionality="1" translationMW="10290.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="4781-5062" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="GvpK" directionality="1" translationMW="10316.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5141-5419" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="GvpJ" directionality="1" translationMW="11129.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5498-5794" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GvpU" directionality="1" translationMW="14827.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5873-6283" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="GvpN" directionality="1" translationMW="34696.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="1718-2641" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="GvpF" directionality="1" translationMW="28861.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="2717-3481" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="GvpL" directionality="1" translationMW="31145.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3896-4702" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="EmGFP" directionality="1" translationMW="26897.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6362-6364" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6365-6367" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6368-7081" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Emerald GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7527-7545" color="#ff0000" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7549-7561" color="#ffcc99" type="standard"/></Feature><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7546-7548" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>a596e1e3-5f8f-4867-a0ce-d4b5ee014535</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:38:55">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></BottomStrand></StrandColors>  _:     
!  'Y        'Y{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èØ|º]TT4�:¦uqTBºµ8NjU¼9¯kj�ãÃ5_º“13£•Q2ø–8cpÜN×]Q©h1ªpÜMUIl}'++‹™UFBx4<hèS‰U`æs†§ZžÊå>1Å–n®lf‘EŽ<â`IU‡Æú¢3#oÆ¯2Û­ÔÖôá&<2fÍF3®ÇB–Ÿb¦†™QÛ1ª\W$QFÌÝh—;$ÕÈÅ|îqWM0zOŒ³¦9þ�sMGkæÆ!Å­\¦&Æj�¶ó§ÕŠ�…cUMSÅU¶©X”Y‘ÞUN…^5ÕªKªTÐÐG,AWøÈa!]\WBjfªLöµpeGZƒ<�IU8Wj¡¬çTs`“NŸU©¶ùlõZšXÛUÍ$Ó`uÂšõ´êbkVÕE•Jê�Wêo4¨U8«ÓTZ°©T–€UQ`èEU%qVø^„T›M¼•AVÑM<)¦&µèX;U©ëøÕQKQÑˆªï:SLÓU5a=p1\cªW2êéi|ES¨aU%aLØUàTuO­§L/ øé–¨…ÚÏ•IEMø;1õë¶žÕÔðG(Aô?ˆãu;!óÿm~!FÃB4CÇSN'�ßoMá½8Ñ†+€Äoñ>½TAÅã†œFÏSÄhM ^ÑLT×4Eh\×V©.©SCA±_à!€E§‰©œ*ZõÕE4ÓÙÊ½ºe ¨pEÚÞ'õ£*B8’®S�Žù­RÔUXA˜N4SjÛmÅp“Î¢qE©ÆÚö•j�ö¦T!EK�9¡]QTdE­áÎ:Ô…ˆU©âgDéUAm1\XTÈTÅUÔV’Þ›}*¨UU‘´á¡U‘:ÝUÕ"!DgaÔåQi³©‡¸¥TZÕ—ƒ4át`ÈLŠºïË¸Ñ§”¬•ÅdQ:ÓT:eC�J…!À}ƒÏæÚ»¨ÌŽ·V’1õë¶žÕÔðG(Aô?ˆO…4M�PfÏá±Ûìràu öÔ~næÏ�ø"~á8nôÈþÐõD}Ôý·ÓJÇSED†=ðôý5 uo¸0Mø$Ã=Åª²ŠÔà(QôˆÓò/ÐõÃE½øñ´<]àý;!‡ÈlN8ÃFÈÏaH0ßáñÄÑ±àÏá…øENŠÁàs„…Û€þà4øáÆQƒAuj’ê•44ËUþò ]×cDÓ}²#�To�Ô²<Ût€[<rã‡ÛQ¼8lƒÓ uTVÃ`h qÿŸ„ÓP3×]C¸AWQŽ7w�DQ±S9‘Löƒ1õë¶žÕÔðG(Aô?ˆMðÿN´ßPußàYë!CÓ]MÃ©>Çm>!`!¤Ã}†Ó=Q'¬yøLGk@Óƒô?‹„Ò¤ø91¼K–SÄ?zÛß…}FçŒ1ÓQ7ÿM@Ôn¸3FÏ�Á®¾U´Ç=KßME/NÚÕÇ±Ïqj¬¢…58ŠEýâ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷è~pÈÒ:ÏÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷P¹›|ñÔÑEÃmB­GóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IE®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¥¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  µ  
_  
œ  ˜  é  ú  7  o  +  <  ¢  ³  ‹  œ   ˆ       ®ZTR

BASE       i  xÁA  ƒ°Yãx` þµ,9ImM¥,	¦èŠ©1)¤Â•JÎm1#—Ë’˜­ÀSA´‚²D]hIÌæ*˜©‹º‚b…‘ˆZ›…+L£Ð2CW©s•¤ËµxªˆMk   ƒ<AlignableSequences trimStringency="Medium"><Sequence name="GVPK" ID="0" sortOrder="1" trimmedRange="0..281"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Oâ<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7134-7255" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8076-8092" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8052-8068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8145-8166" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8100-8106" color="#ffffff" type="standard"/><Segment range="8107-8124" color="#ffffff" type="standard"/><Segment name="-35" range="8125-8130" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10004" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9213-10004" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10005-10073" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8454-9042" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6355" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="6302-6358" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5866" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5813-5869" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5491" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5438-5494" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5134" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5081-5137" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4774" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="4721-4777" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3889" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3836-3892" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3550" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3497-3553" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2710" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="2657-2713" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="GvpG" directionality="1" translationMW="10440.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3557-3817" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="GvpS" directionality="1" translationMW="10290.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="4781-5062" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="GvpK" directionality="1" translationMW="10316.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5141-5419" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="GvpJ" directionality="1" translationMW="11129.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5498-5794" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GvpU" directionality="1" translationMW="14827.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5873-6283" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="GvpN" directionality="1" translationMW="34696.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="1718-2641" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="GvpF" directionality="1" translationMW="28861.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="2717-3481" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="GvpL" directionality="1" translationMW="31145.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3896-4702" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="EmGFP" directionality="1" translationMW="26897.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6362-6364" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6365-6367" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6368-7081" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Emerald GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7527-7545" color="#ff0000" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7549-7561" color="#ffcc99" type="standard"/></Feature><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7546-7548" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>a596e1e3-5f8f-4867-a0ce-d4b5ee014535</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:38:49">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></BottomStrand></StrandColors>  _:     
!  'Y        'Y{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èØ|º]TT4�:¦uqTBºµ8NjU¼9¯kj�ãÃ5_º“13£•Q2ø–8cpÜN×]Q©h1ªpÜMUIl}'++‹™UFBx4<hèS‰U`æs†§ZžÊå>1Å–n®lf‘EŽ<â`IU‡Æú¢3#oÆ¯2Û­ÔÖôá&<2fÍF3®ÇB–Ÿb¦†™QÛ1ª\W$QFÌÝh—;$ÕÈÅ|îqWM0zOŒ³¦9þ�sMGkæÆ!Å­\¦&Æj�¶ó§ÕŠ�…cUMSÅU¶©X”Y‘ÞUN…^5ÕªKªTÐÐG,AWøÈa!]\WBjfªLöµpeGZƒ<�IU8Wj¡¬çTs`“NŸU©¶ùlõZšXÛUÍ$Ó`uÂšõ´êbkVÕE•Jê�Wêo4¨U8«ÓTZ°©T–€UQ`èEU%qVø^„T›M¼•AVÑM<)¦&µèX;U©ëøÕQKQÑˆªï:SLÓU5a=p1\cªW2êéi|ES¨aU%aLØUàTuO­§L/ øé–¨…ÚÏ•IEMø;1õë¶žÕÔðG(Aô?ˆãu;!óÿm~!FÃB4CÇSN'�ßoMá½8Ñ†+€Äoñ>½TAÅã†œFÏSÄhM ^ÑLT×4Eh\×V©.©SCA±_à!€E§‰©œ*ZõÕE4ÓÙÊ½ºe ¨pEÚÞ'õ£*B8’®S�Žù­RÔUXA˜N4SjÛmÅp“Î¢qE©ÆÚö•j�ö¦T!EK�9¡]QTdE­áÎ:Ô…ˆU©âgDéUAm1\XTÈTÅUÔV’Þ›}*¨UU‘´á¡U‘:ÝUÕ"!DgaÔåQi³©‡¸¥TZÕ—ƒ4át`ÈLŠºïË¸Ñ§”¬•ÅdQ:ÓT:eC�J…!À}ƒÏæÚ»¨ÌŽ·V’1õë¶žÕÔðG(Aô?ˆO…4M�PfÏá±Ûìràu öÔ~næÏ�ø"~á8nôÈþÐõD}Ôý·ÓJÇSED†=ðôý5 uo¸0Mø$Ã=Åª²ŠÔà(QôˆÓò/ÐõÃE½øñ´<]àý;!‡ÈlN8ÃFÈÏaH0ßáñÄÑ±àÎá…øENŠÁàs„…Û€þà4øáÆQƒAuj’ê•44ËUþò ]×cDÓ}²#�To�Ô²<Ût€[<rã‡ÛQ¼8lƒÓ uTVÃ`h qÿŸ„ÓP3×]C¸AWQŽ7w�DQ±S9‘Löƒ1õë¶žÕÔðG(Aô?ˆMðÿN´ßPußàYë!CÓ]MÃ©>Çm>!`!¤Ã}†Ó=Q'¬yøLGk@Óƒô?‹„Ò¤ø91¼K–SÄ?zÛß…}FçŒ1ÓQ7ÿM@Ôn¸3FÏ�Á®¾U´Ç=KßME/NÚÕÇ±Ïqj¬¢…58ŠEýâ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷è~pÈÒ:ÏÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷P¹›|ñÔÑEÃmB­GóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IE®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¥¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí  µ  
_  
œ  ˜  é  ú  7  o  +  <  ¢  ³  ‹  œ   ˆ       ®ZTR

BASE       i  xÁA  ƒ°Yãx` þµ,9ImM¥,	¦èŠ©1)¤Â•JÎm1#—Ë’˜­ÀSA´‚²D]hIÌæ*˜©‹º‚b…‘ˆZ›…+L£Ð2CW©s•¤ËµxªˆMk   ƒ<AlignableSequences trimStringency="Medium"><Sequence name="GVPK" ID="0" sortOrder="1" trimmedRange="0..281"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Oâ<?xml version="1.0"?><Features nextValidID="43"><Feature recentID="42" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="41" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="40" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="39" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="38" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="37" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="36" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="34" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7134-7255" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8076-8092" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8052-8068" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8145-8166" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8100-8106" color="#ffffff" type="standard"/><Segment range="8107-8124" color="#ffffff" type="standard"/><Segment name="-35" range="8125-8130" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10004" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9213-10004" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10005-10073" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8454-9042" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6355" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="6302-6358" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5866" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5813-5869" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="22" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5491" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5438-5494" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5134" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="5081-5137" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="20" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4774" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="4721-4777" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3889" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3836-3892" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3550" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="3497-3553" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="P2A" directionality="1" translationMW="1987.15" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2710" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081" detectionMode="exactProteinMatch"><Segment range="2657-2713" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from porcine teschovirus-1 polyprotein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="GvpG" directionality="1" translationMW="10440.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3557-3817" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="GvpS" directionality="1" translationMW="10290.87" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="4781-5062" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="GvpK" directionality="1" translationMW="10316.79" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5141-5419" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="GvpJ" directionality="1" translationMW="11129.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5498-5794" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="GvpU" directionality="1" translationMW="14827.42" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="5873-6283" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="11" name="GvpN" directionality="1" translationMW="34696.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="1718-2641" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="GvpF" directionality="1" translationMW="28861.93" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="2717-3481" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="GvpL" directionality="1" translationMW="31145.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="7081" maxFusedRunOn="7081"><Segment range="3896-4702" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="EmGFP" directionality="1" translationMW="26897.45" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6362-6364" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6365-6367" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6368-7081" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Emerald GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7527-7545" color="#ff0000" type="standard"/></Feature><Feature recentID="2" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7549-7561" color="#ffcc99" type="standard"/></Feature><Feature recentID="0" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7546-7548" color="#a6acb3" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>a596e1e3-5f8f-4867-a0ce-d4b5ee014535</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:38:42">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..6360" colors="black"/><ColorRange range="7081..10072" colors="black"/></BottomStrand></StrandColors>  /      ´  …         …{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èÀüÃÏðËf®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¥¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &X<?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3122-3710" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4672" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="3881-4672" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="4673-4741" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="2768-2774" color="#ffffff" type="standard"/><Segment range="2775-2792" color="#ffffff" type="standard"/><Segment name="-35" range="2793-2798" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2813-2834" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2720-2736" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2744-2760" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1802-1923" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1712-1755" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:11:18">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..4740" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..4740" colors="black"/></BottomStrand></StrandColors>  /z     .  o         o{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èÀüÃÏðËf®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &X<?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3612-4200" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5162" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4371-5162" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5163-5231" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3258-3264" color="#ffffff" type="standard"/><Segment range="3265-3282" color="#ffffff" type="standard"/><Segment name="-35" range="3283-3288" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3303-3324" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3210-3226" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3234-3250" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1802-1923" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1712-1755" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:10:30">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5230" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5230" colors="black"/></BottomStrand></StrandColors>  0     M  é         é{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’©ïæÒraD©ïæÒrcG©ïæÒre©ïæÒraô©ïæÒrnt©ïæÒrfô©ïæÒrbÄ$2'ù•M²¤…òÑ³àMkÝwª.™èÀüÃÏðËf®~s˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¥z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹�h{Vµ¤¹´S›‚ïëÀQÛø©f¥tÒ+õx
é›ú;‚×Ð£@«âût¿•Ò=
“ü‡�¯ª¤Ú	ƒª¿É¿ý¸ÐíQ4ÄS+D1¿#ß¯ò?‹üÜãÃ°0 Lð|ÿÎ8ÿäÀ%§oà¨  âþ¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &i<?xml version="1.0"?><Features recycledIDs="1" nextValidID="20"><Feature recentID="18" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1712-1755" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1370-1388" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1406-1424" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1478-1496" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1514-1532" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1550-1568" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1586-1604" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1802-1923" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3356-3372" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3332-3348" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3425-3446" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3380-3386" color="#ffffff" type="standard"/><Segment range="3387-3404" color="#ffffff" type="standard"/><Segment name="-35" range="3405-3410" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5284" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4493-5284" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5285-5353" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3734-4322" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  =<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:1:6">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..1363" colors="black"/><ColorRange range="1923..5352" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1363" colors="black"/><ColorRange range="1923..5352" colors="black"/></BottomStrand></StrandColors>  0Ê     P  ÷         ÷{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’§¿›HdEÉ…§¿›HdEÉ�§¿›HdEÉ”§¿›HdEÉ‡Ò§¿›HdEÉ¹Ò§¿›HdEÉ›Ò§¿›HdEÉ‹�Èœ 3æU4NÊ’ËFÏ�5¯uÞ¨ºg¡ûÙk‘91k¥lÌ<ç9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºW¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ')<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="19" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1816-1937" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1731-1773" color="#ffff99" type="standard"/></Feature><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3370-3386" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3346-3362" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3439-3460" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3394-3400" color="#ffffff" type="standard"/><Segment range="3401-3418" color="#ffffff" type="standard"/><Segment name="-35" range="3419-3424" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1589-1607" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1553-1571" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1517-1535" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1481-1499" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1445-1463" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1409-1427" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1373-1391" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1714" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5298" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4507-5298" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5299-5367" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3748-4336" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1938-2059" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="6:0:25">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..1713" colors="black"/><ColorRange range="1937..5366" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1713" colors="black"/><ColorRange range="1937..5366" colors="black"/></BottomStrand></StrandColors>  0Ç     L  ç         ç{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’§¿›HdEÉ…§¿›HdEÉ�§¿›HdEÉ”§¿›HdEÉ‡Ò§¿›HdEÉ¹Ò§¿›HdEÉ›Ò§¿›HdEÉ‹�Èœ 3æU4NÊ’ËFÏ�5¯uÞ¨ºg«‘91k¥lÌ<ç9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºW¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ')<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="19" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1800-1921" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1715-1757" color="#ffff99" type="standard"/></Feature><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3354-3370" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3330-3346" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3423-3444" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3378-3384" color="#ffffff" type="standard"/><Segment range="3385-3402" color="#ffffff" type="standard"/><Segment name="-35" range="3403-3408" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1589-1607" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1553-1571" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1517-1535" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1481-1499" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1445-1463" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1409-1427" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1373-1391" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1714" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5282" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4491-5282" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5283-5351" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3732-4320" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1922-2043" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:58:39">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..1713" colors="black"/><ColorRange range="1921..5350" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1713" colors="black"/><ColorRange range="1921..5350" colors="black"/></BottomStrand></StrandColors>  /i     #  C         C{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’§¿›HdEÉ…§¿›HdEÉ�§¿›HdEÉ”§¿›HdEÉ‡Ò§¿›HdEÉ¹Ò§¿›HdEÉ›Ò§¿›HdEÉ‹�Èœ 3æU4NÊ’ËFÏ�5¯uÞ¨ºg«‘91k¥lÌ<×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %ô<?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1715-1757" color="#ffff99" type="standard"/></Feature><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3190-3206" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3166-3182" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3259-3280" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3214-3220" color="#ffffff" type="standard"/><Segment range="3221-3238" color="#ffffff" type="standard"/><Segment name="-35" range="3239-3244" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1589-1607" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1553-1571" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1517-1535" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1481-1499" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1445-1463" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1409-1427" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1373-1391" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1714" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5118" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4327-5118" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5119-5187" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3568-4156" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1758-1879" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:58:14">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="0..1713" colors="black"/><ColorRange range="1757..5186" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1713" colors="black"/><ColorRange range="1757..5186" colors="black"/></BottomStrand></StrandColors>  .P     !  ;         ;{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’§¿›HdEÉ…§¿›HdEÉ�§¿›HdEÉ”§¿›HdEÉ‡Ò§¿›HdEÉ¹Ò§¿›HdEÉ›Ò§¿›HdEÉ‹�Èœ 3æU4NÊ’ËFÏ�5¯uÞ¨ºg Ÿ˜ØòS­"—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3182-3198" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3158-3174" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3251-3272" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3206-3212" color="#ffffff" type="standard"/><Segment range="3213-3230" color="#ffffff" type="standard"/><Segment name="-35" range="3231-3236" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1589-1607" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1553-1571" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1517-1535" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1481-1499" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1445-1463" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1409-1427" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1373-1391" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1714" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5110" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4319-5110" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5111-5179" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3560-4148" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1750-1871" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:57:33">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5178" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5178" colors="black"/></BottomStrand></StrandColors>  .R     #  B         B{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚~c`oÉN´Š^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3189-3205" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3165-3181" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3258-3279" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3213-3219" color="#ffffff" type="standard"/><Segment range="3220-3237" color="#ffffff" type="standard"/><Segment name="-35" range="3238-3243" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5117" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4326-5117" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5118-5186" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3567-4155" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1757-1878" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="5:55:54">2021.4.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5185" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5185" colors="black"/></BottomStrand></StrandColors>  .¾   
  #  C         C{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚~c`òS­"—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1758-1879" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3568-4156" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5118" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4327-5118" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5119-5187" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3214-3220" color="#ffffff" type="standard"/><Segment range="3221-3238" color="#ffffff" type="standard"/><Segment name="-35" range="3239-3244" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3259-3280" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3166-3182" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3190-3206" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:49:31">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5186" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5186" colors="black"/></BottomStrand></StrandColors>  .¿   	  $  H         H{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚~ccßü”ëH¥è©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.¡íXbÖ’æÑNn¿¯ Goâ¥š•ÓH¯Õà+¦oèî_B�¯‹íÐ\RþTWH4ô*OðZ¾ª�?h&ªÿ&ÿô"ãCµDÓL­TÆü�~¿Èü/ós�ÀÀ3ÁðLü;8ãÿ“ –�¿€  ‹øfæ]EY™™e)·%$]…eÖe¦É„¥n•KrUmŒ¶¨{s)’µ[Hw§Í¡ÔÜûp½L1á¾V7Lpk3š‘Q5šµ62Ö©Ín®@¥¹8ÛA¶l°.ª¼;KlýNæà6ÀQOÈú¯ÌB…0Ø£Äxã�¡Êsr§Øa¬ƒØÜú—^++Cß€gÒ²Ì:¯t¯OÅ3m5ü]gÃ<�#Â#F–0Œ3€fœ´Ä	¾<ÑX�ŸòŸÃ3 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1763-1884" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3573-4161" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5123" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4332-5123" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5124-5192" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3219-3225" color="#ffffff" type="standard"/><Segment range="3226-3243" color="#ffffff" type="standard"/><Segment name="-35" range="3244-3249" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3264-3285" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3171-3187" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3195-3211" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:49:28">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5191" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5191" colors="black"/></BottomStrand></StrandColors>  .Á     &  O         O{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚~ccßÂ<òS­"—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1770-1891" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3580-4168" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5130" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4339-5130" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5131-5199" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3226-3232" color="#ffffff" type="standard"/><Segment range="3233-3250" color="#ffffff" type="standard"/><Segment name="-35" range="3251-3256" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3271-3292" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3178-3194" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3202-3218" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:49:18">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5198" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5198" colors="black"/></BottomStrand></StrandColors>  .¾     #  B         B{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚ðoÉN´Š^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1757-1878" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3567-4155" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5117" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4326-5117" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5118-5186" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3213-3219" color="#ffffff" type="standard"/><Segment range="3220-3237" color="#ffffff" type="standard"/><Segment name="-35" range="3238-3243" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3258-3279" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3165-3181" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3189-3205" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:48:50">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5185" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5185" colors="black"/></BottomStrand></StrandColors>  .¼     !  <         <{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚ðoËH¥è©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.¡íXbÖ’æÑNn¿¯ Goâ¥š•ÓH¯Õà+¦oèî_B�¯‹íÐ\RþTWH4ô*OðZ¾ª�?h&ªÿ&ÿô"ãCµDÓL­TÆü�~¿Èü/ós�ÀÀ3ÁðLü;8ãÿ“ –�¿€  ‹øfæ]EY™™e)·%$]…eÖe¦É„¥n•KrUmŒ¶¨{s)’µ[Hw§Í¡ÔÜûp½L1á¾V7Lpk3š‘Q5šµ62Ö©Ín®@¥¹8ÛA¶l°.ª¼;KlýNæà6ÀQOÈú¯ÌB…0Ø£Äxã�¡Êsr§Øa¬ƒØÜú—^++Cß€gÒ²Ì:¯t¯OÅ3m5ü]gÃ<�#Â#F–0Œ3€fœ´Ä	¾<ÑX�ŸòŸÃ3 Ém‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="10" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3183-3199" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3159-3175" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3252-3273" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3207-3213" color="#ffffff" type="standard"/><Segment range="3214-3231" color="#ffffff" type="standard"/><Segment name="-35" range="3232-3237" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5111" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4320-5111" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5112-5180" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3561-4149" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1751-1872" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:45:28">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5179" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5179" colors="black"/></BottomStrand></StrandColors>  .»        6         6{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚ü´Š^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  %;<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="10" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3177-3193" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3153-3169" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3246-3267" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3201-3207" color="#ffffff" type="standard"/><Segment range="3208-3225" color="#ffffff" type="standard"/><Segment name="-35" range="3226-3231" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5105" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4314-5105" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5106-5174" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3555-4143" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1745-1866" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:45:16">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..5173" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..5173" colors="black"/></BottomStrand></StrandColors>  %[     &  N         N{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚|<ÔŒlïü´Š^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Ó<?xml version="1.0"?><Features recycledIDs="0,4,7,8,10" nextValidID="18"><Feature recentID="17" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5129" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4338-5129" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5130-5198" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3579-4167" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1769-1890" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:41:16">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   «<StrandColors><TopStrand><ColorRange range="1364..1726" colors="red"/></TopStrand><BottomStrand><ColorRange range="1364..1726" colors="red"/></BottomStrand></StrandColors>  1Ì     $  G         G{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚|<ÔŒlïü´€Ñ3vÏÝ/ñ  @,5¡p#äE3 •à!‹"x;Ïª¿ É™HÒK<…Êê¬×£ÏEÝ8S¬@;6îúÜÏÏ?Ÿ„<ö÷<(K2¸óïÏˆ#ï…ã/<¹	J•;BÄ|¨¼3¿ OŸ‘í<;½Î£áþ:;^ç.¢¬ªâ¸Ï)Ñ½N!ðÏœäsß`DÇ0øüölHómðßápÈ±8 0Á7ÿS@óaõÔaDßT3ø~´ÿŸŽÜ†œ:çÞ°ßŸôÃœEÿ¯�ÏmuÃw×6ÄRq28Ü„ëN|çðó!°Ç­BðëüAÓ!ºßFÛÍ5Ï|�7ãØeÈà€Ó¬üß­>30ç�:È t÷czÔLö÷ý6øÑpýŠÏ¯<ÈN7MwÄà6È}7>µý³ë=ó;K/ZãÈ7Ïðï|³4AK®]Œ!ÈE¿æu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>µû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&Ò—¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸‡µa‹ZK›E9¸.þ¼ u¿Š–jWM"¿W€®™¿£¸-}
4
¾/·AqKùQ] ÓÐ©?Áhxúª@ý ˜:«ü›ÿÐ‹�ÕLE2´ASò=úÿ#ð8¿ÍÎ<; c ÏÁ0Oð0ìã�þL @rZvþ :€ /á›™uffe”¦Ü”‘v—Y–›&•ºU-ÉTe¶2Ú¡íÌ,¦JÕm!ÞŸ6‡SsíÀNõ0Ç„bùXÝ1Á¬ÎjEDDÖjÔXØËZ§4yº¹–äãmÙ²Àºªðí-³õ83›€Û AE?#ê¿1
Ã`TJ�aãŽ6‡(LUÍÊŸa†°"csê\!x¬­~ŸJË0ê¼Ò¼-?Í´×ñuŸtð^<��XtÂ0ÎšrÓp&ø8óE`vÈJÎƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…ž6   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &G<?xml version="1.0"?><Features recycledIDs="10" nextValidID="18"><Feature recentID="17" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7170" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6379-7170" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7171-7239" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="3702" maxFusedRunOn="3702"><Segment range="2317-2979" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3103-3702" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5620-6208" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1766-1977" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3810-3931" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3099" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="3702" maxFusedRunOn="3702" detectionMode="exactProteinMatch"><Segment range="3049-3102" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ª<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:41:7">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   «<StrandColors><TopStrand><ColorRange range="1364..1726" colors="red"/></TopStrand><BottomStrand><ColorRange range="1364..1726" colors="red"/></BottomStrand></StrandColors>  1Í     %  I         I{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚|ÃÍA¸ÆÎð�ËH7lýÒÿJ ÃZ>DPƒ0	^R²'ƒ¼ú«ñ |™”�$³È\®ªÍp*<ô]Ó…:Ä³nï­ÌüóùøCÏosÂ„³+�>üø‚>ø^2óË�”©S´,GÀú‹Ã;úùùÓÃ»Üê>ã£µîrê*Ê®+Œò�ÔâùÎG=öLq�Ï`FÄ�6ßþ�+‚õ46]FMõC?‡ëOùøá=È`9Ã®}ëùÿL9Ä_úøÜö×\0ç}wólE'pÃ#�ÈN´çÎq2zÔ €ß¿Ä2­ñÔm¼Ó\÷È~7ý€Æ\Ž:ÏÍúÓã1ÃyÓ¬‚Ov7­DÏoÓo� ?Ø¬úóÌ�ãt×|Nl�'ÓsëQÿÛ>³Ñ¿3´²õ®<�Ã|ð÷Á»1DºåØÂ�[ðþañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#á[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢m)z*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹�h{Vµ¤¹´S›‚ïëÀQÛø©f¥tÒ+õx
é›ú;‚×Ð£@«âût¿•Ò=
“ü‡�¯ª¤Ú	ƒª¿É¿ý¸ÐíQ4ÄS+D1¿#ß¯ò?‹üÜãÃ°0 Lð|ÿÎ8ÿäÀ%§oà¨  âþ¹—QVffYJmÉIaYu™i²a)[¥RÜ•F[c-ªÜÂÊd­VÒéóhu7>ÜïSxF/•�ÓÌæ¤TDMf­E�ŒµªsG›«�)nN6Ðm›,ª¯ÒÛ?Sƒ9¸°Sò>«ó¡L6D¨ñ8ãhr„Å\Ü©ökB ö7>¥ÂŠÊÐ÷àô¬³«ÁÝ+Á‚ÓñLÛMYðÇOãÈðˆÑ¥‡L#à§-1oƒ��4V`gü„§ðÀÌè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘AÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yí   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &G<?xml version="1.0"?><Features recycledIDs="10" nextValidID="18"><Feature recentID="17" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7172" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6381-7172" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7173-7241" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="3704" maxFusedRunOn="3704"><Segment range="2319-2981" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3105-3704" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5622-6210" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1768-1979" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3812-3933" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3101" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="3704" maxFusedRunOn="3704" detectionMode="exactProteinMatch"><Segment range="3051-3104" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ª<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:39:7">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   «<StrandColors><TopStrand><ColorRange range="1364..1726" colors="red"/></TopStrand><BottomStrand><ColorRange range="1364..1726" colors="red"/></BottomStrand></StrandColors>  1Ñ     (  V         V{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’­ìJžþm!‘&Jžþm!‘&4zžþm!‘&PJžþm!‘&Jžþm!‘&çJžþm!‘&oJžþm!‘&,BC"p€Ï™TÑ;*H_->Ö½×z¢éž‚~ccß0óPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›J^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &G<?xml version="1.0"?><Features recycledIDs="10" nextValidID="18"><Feature recentID="17" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1596-1614" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1560-1578" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1524-1542" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1488-1506" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1470" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1416-1434" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1380-1398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1372-1721" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-OnÂ® 3G, optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7185" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6394-7185" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7186-7254" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="3717" maxFusedRunOn="3717"><Segment range="2332-2994" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3118-3717" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5635-6223" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1781-1992" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3825-3946" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3114" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="3717" maxFusedRunOn="3717" detectionMode="exactProteinMatch"><Segment range="3064-3117" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:38:56">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   «<StrandColors><TopStrand><ColorRange range="1364..1739" colors="red"/></TopStrand><BottomStrand><ColorRange range="1364..1739" colors="red"/></BottomStrand></StrandColors>   é      )  Z         Z{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷åh”ÊYª)V³)Z¨¥m;j©}d<VÃU¿¦VÕE‘ñ„Š+Ò ]D½æ• {×-P1UòæÐ1ƒ÷œ…ößåµ*¨Ä#ÉSy[þTÿÆ¤N‡ TðÅÈ1ELT985"L-Î3%÷Ýüó:XÏ9Ð3/ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃÒ3ì­­Ê¨_Èp4{0�XŠ§L†Ñ…ìw8Ñw4äi‘Q–Ù¢œ¥F[c%ZØŠ¦ÂâfÄ*jijFiJ¦™©Þ�žYek]]jjb¦¦¦•U]UíV®¹•%Õz¦?ÿõOý‰iËûÿ9 4ÓÏÀ;Îð¼3¿üÛüO20OÀÜBÀlêû…Î»8î¡>4wà g U½Cç’i�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQ®DäÅ¬Z•°o0óPn1³¼#òÒDÍÛ?t¿ÄR€ °Ö…À�‘ ÌW€T†,‰àï>ªü@€_&e#I,ò+ª³\
�=táN± ìÛ»ës?<þ~óÛÜð¡,ÊãÏ¿> �¾Œ¼òä%*Tíð>¢ðÎþ�>~G´ðî÷:�‡øèí{œºŠ²«Šã<§Fõ8‡Ã>s‘Ï}�DÃãóØ±#Í·Ã…Ã JÄà€Ã@ßýMÍ‡×Q…}PÏáúÓþ~8OrpëŸzÃ~Óqþ¾7=µ×9ß]üÛIÄ0Èãr­9óœCÃÌ†Ãµ 7Ã¯ñL†ë|uo4×=òß�ÿ`1—#‚N³ó~´øÌpÃžtë �ÓÝ�ëQ3ÛßôÛãEÀö+>¼ó A8Ý5ß€Û IôÜúÔöÏ¬ôoÌí,½k� pß<Ã½ðnÌ@Ñ.¹v0‡ Aü?˜|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øV×î�V¢	®—˜£¾©ÎbsŽ¥�¨›J^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàZÕ†-i.mæà»úðÔvþ*Y©]4Šý^ºfþŽàµô(Ð*ø¾ÝÅ/åEtƒOB¤ÿ¡àkê©ö‚`ê¯òoÿB.4;TM1ÊÑLoÈ÷ëü�Àâÿ78ðìŒ <Á?ÀÃ³Ž?ù0ÉiÛø ê  8¿†neÔU™™–R›rREØV]fZl˜JVéT·%Q–ØËj‡·0²™+U´‡z|ÚMÏ·;ÔÃ‹åctÇ³9©Y«Qcc-jœÑæêä
[“�´fËê«Ã´¶ÏÔàÎnlü�ªüÄ(S�Q*<A‡Ž8Ú¡1W7*}†ÀPˆ=�Ï©p…â²´=ø}+,ÃªðwJð`´üS6Ó_ÅÖ|1ÓÁxò<"4iaÓÃ8iËLAÀ›ààcÍ�Øÿ!)ü03:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äPtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1710-1752" color="#ffff99" type="standard"/></Feature><Feature recentID="9" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7189" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6398-7189" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7190-7258" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="CopGFP" directionality="1" translationMW="24522.73" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="2" maxRunOn="3721" maxFusedRunOn="3721"><Segment range="2336-2998" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/14963095&quot;&gt;Shagin et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3122-3721" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5639-6227" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="EF-1Î± core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1785-1996" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1Î±&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1442-1645" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3829-3950" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3118" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="3721" maxFusedRunOn="3721" detectionMode="exactProteinMatch"><Segment range="3068-3121" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  «<Notes>
<UUID>9cf87773-5fac-4b09-8d22-99612f8dbcf4</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac Dual promoter (PB513B-1)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="8:38:25">2021.2.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Pý7zXZ  æÖ´F !   t/å£ã“»] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷¿Üµ¡&õý{Îb¯Ác�õÚÝR¦äÌƒžÁé»ïE¦®Öš1££°ûÅPÔäUÿÐê½Híl’hø#­±Z�ïÑ�èÆÊE…{šcñmõ4åVÈ©É@¢‹E.¥„ÈU7Y}âÏÃ ÇkðV6-˜¥Ô
‘¾BÐTôOð^ðï¿ä;Ïñt©Sõ“ˆ¥ÅPáð'ÚÀCæ—ù$àÃ?–Ü¼Â|o&ê×‹Oˆˆ#·Qäçlô´ŠB!n�²tÐ}0s3nâÌmÈ–¡HoÁX9-®^ì9�zš`€ÌÕ)U5,+û¾ÙÏ,y8ñë–{s'ó”Üzvötñ>�:µßŠ�áƒ™²d•˜Û/3zR�Ê6AG¥U³�=	®,~`ÜÀDÿÉwKY«ÆB‘Àapç‹ž^çðMž:�a€Î"T-ôú$Éî—ôŠH  >P
±rî@q˜Ž¨pèb´�Ò«ænÏ38bzløÌþD Ú-ÌsAŸ/ ÕÑ9üEQÂ™’ß'ˆ*Æ¨U’ØkÏGþE¶ÈÍ¤á)éõXùñÀšÆ	8ŸmžàWLåªã$%QÃ}�°4×œÿ,žw´¥‚öètä¨Ž¸öÂª½-÷Œš÷�úE­˜_”UÄ ž&bŠz­åyµYÜäúmÇ¿tdSØ·\(®ñ¬^Æq®â2kÉ‰*Yƒ!¯Þ¨¢Cƒ}hl|)Œ8³#=M?“,3 ¾ÌÉ£yX„D‘g“ ºˆ©î”YQí„¦ÑVÜ_/ü[÷”‘Ñb6Ê"SAâ.`Œ9ÏCÎ6�^°Îï=1rtrØ$@R4!‰Ú£¤-xèx·‡s´äBò5´s¤d°;M£:™Izgt¯µ“S&ó><~ÿmjÙ¼Í[ä9=¶PÒF‰ÙpŠÌ4°wÝ9BVáßáÃÊÿ&÷®ž¸Ñ¯ÇšÙ"è¢¹!N1ê~iøÎe§ í/AšËWY?Ú‹À,Á×ž+ô˜Ý–ú1v� \*+Ù²2²½V,YÏÊ¬ûB�¯>›Úƒã2Ÿ¹ªcYhñäÉÍ²0šÉ;ðIŒÈ\wQð°k¬¿„'¢‘Vrå—ÐÊ=�]Ö2¾ô€¼«ÿÍØÓ‰Ï�Íùµø|6©þ"=ÂöY—ÒÞsß68h<ŸT•ä7Ní¡÷‚Ë[“6Y<Gþkd4ÚlÍñUÑYi!ókÌ}Q1	œikÕ›î`}†ŠH�”ÕV>=é÷Î fåýzÁ!âOI	±–^t(¯ããæé:¯9)ÉÌs5ê³e
èˆ‰‘1Ìœ`ÞJÚü±S‰æzÖ¸�	š÷õBAbCõ¬ÛiByá†7&¶Ÿº¦Wâ\nnYûµ§A·2ë¡[’rÂòêkìÅ\'ZÏÇÚâÂWPY©´*U©˜x¿_uä#ÍNª±�ÏïŠú¼°¼"÷RŠŽr%ûéºäPþ7€#\G·†TVÑ"gLÈ toxÊÙ€q/x*˜cJ›Q`@R.\hê”a8ç÷t�Þqµ¯Å®¢ ¢490oS©_­èßã°Ü‹ô9t
�½©¦ÃlCR”È«Mß¤š\ÉFE¶BàäÖà¬»µnÜªÛË�i"ÀK#°¶¡¤M“)„�v—uk«kÛb¦28•jó(> Ò~fº,ŸsßT uó%åÃß:€‡šÆv[ù[Hõ¶tgÝSfÇâe×ÈçÐ’º}úáWoRu³öÏ¬¢;žó¯36µAHã�»×°jS ïa„XMœéÒõ�è ï¨T'
whèÝ£áÀ"å£rY$±w?bj�aaTáK­<)„(µ™n}2Ž&õ÷¹:®ígDgºàì]bí;‘ñ"Hë[7‹ß×é% 1Œ:áùz]685ƒ Òq6ÊdÏ	KäZ'F÷ÜQÌÌ«õÙë‹§š_C^–nTŸ—Ä+)–RÄsîtlØä­¹êDÒ>°=‘ƒšíE¯¬¬ñÔ÷Ò6¢]:Î¼q)ŒØÁ²Qc~AX4åm6€
,º½KCÊ
){°Ïªp+"6X%d‹“í0!™£ÑkµvÕÄÌ°Ø%¨­©çV„ÉFRð73ÖtŽÉˆœ\UAUguÎ®ŸÍ§²VNó¼a\à=„ñF�»†Ã0kC¼t€§¤AßnXÂ4ïÿ†¸QÔ‰ÈÆ$HÞ„WôµŸž2C
Î‹ÊÇ<YÌ­|…SgÆË“0„0B·šJDF_u¨žÚi,ÏâV‹…õñ_.#Çœ‚›Kïcó=Ù8°¥”£O¦	wõ.²À<Àtð@"¶ßV`Rª4L…f2hœGšþYº�ìä\õ.ç«vz�çò§°ß.Y\SvÏ[hÛ‘¯èñvòžËlï>SãSÌ-Á$œÞ—^`AÓÕù�žØˆê+XÀ|É2¸‘Íî,�Ø±ùI´¤Ù½ü±a.fcMÒªÇéÆDG�b`;iC?Dþ—�ü-(W•ãLÂ¢1ÞÔ?£-š#ÑÊÈ~`y* ÝÄÕ}‰ÇÓŠ‘ª<¼ª–Ã"s*÷¿òT-`sëŽyðœZ£B'â}ë—®ö
ÔW
úîP�"_g³ÕƒÝ/NØÎ \]sl´"*XØëwe­wØÓª¿óhD��bH'úò=<êŽéþZQnw²kãGL¸äQv×ã€‹íÉ¯’keÂ®†XƒºÊâÝøbd§©Aè-±(‘¼ÿµ�•¹0ç€ŸawtÛ1ðWÍü›hïh©¤
÷Êå~({ÆGz$©=ä }º¯+i›yaüµë;ÀŠÌGõÕšÃy|Ûá�¤ÕŸ
jM!ÙÚyÿ’xü¯%X˜Ð´•«~�­2KÆÒ	¬váJJdBÙv«Ö¢BŽáKY™~Í¤1l7DŠ�¦¸Ö»3<{]/XrèBòþr©,Ë÷†˜Íï¶³SÀÒŒìÝj(ˆíy	¥Eõ¨Q[¾°Q-Â/ÛÙ¢{*(>žvP´½6Eá7¡©zEtË2*_bÅg³Ài–ËBÓi5c×COI”Všˆ¯ZìÇæ¦íWMƒ.W“À¸”�Ï•œï�)Õ3ÞŽÄQ¡›L~î…Nåó#@(’ÒLÊ9ò&âPdšu%fÒEþü¬ Ú¬¿]ßŠ<ðýª#÷ëÞðh/¹Y\MlFx@bQa-p'wå°ÇÓ‚Œ¬µ1Å�D¾-ó;?˜¦ÙŸsô¹ä®Øxúƒñï½Ä©ƒôbãZY¢Üi~TþŽ½zHq�¬}yù'ÉÅÔ“Ú'�@�ZÞ‚„”0ô=âWõx’RM%ÁÄëb’íÐâv*‡–DD0Îå˜šeh¸­¸§+2²“¾xvg ‚Úï'?¥¦¿ólY,ï ›0 …v¢àÃaÞì¿Ú Ž)…ÚAðÒ½rŸC‚Hÿ:òÓ¶Å�Gns©vü‰kq@W©Z‡ŒÄ2Ð0\û†ÇÏ¥\H<h‚N‘[“Iµó„Ô–zBã�|¥lŒ]zPUŽù#Žƒö°9'”gŸƒä~2	”L)±Æ{·_¼Ã|°wé�½<‡Ù`”/Ð�[ýPÖã®æ!âªpßø,ØÛýàêo7“ôšQ	n¨¥fƒÊÃÑÞ'›ú+X†íS>ÓúqT�t‰CF0Š;4bÍß}g˜EuÅÒR”Iy¹Ä;¸ZvY§ˆj„ãJÅ–·;{Aç�§ŸÌÀÀEÐ›ãNö~P'Î©4e_Yýøê¸™3æžì�é“ò‡ï‚å/Ä¬ó<fL.+_uA´—^uæië¹ã}<ºU‰åÂxm©60ùe†Ï±¿¤
uÇ¬bŸ~ˆ‰h¡åI­Íð9µ÷C‡;lýªÇ¤(ÁÀŸVEE}f=Ða{‰`]à7~·O×»së†2]?{¬D>…9ÿ!*ÿ�íœYþ~œ!0y(±9TêŸpoªŽ�XtkŠ8PÛ°»*O¼±]?¾F!ÅÏ­’�~VýŽÖ˜*ˆDÅ?âŸ‹ÛG`¢›µ;hÿÂií‰§•Ó8„;KÐT©dµscé{$ø(3Î¨Ý÷à&Ld�vïŸoøw/R¤:h­¤:ÞuŸ¥@¿fmi*çÊêÍ@që4ð¼HX‡S°ñPAâ½�žMÉ°A%NÂ…ÿ&Å§gðvt«`	-†.V1ØPÏq‘ õñ±®æÄÄúê£m¸X"æƒ£š+%sµ<€lº©wI.¡�93ÙeD~þýÔ“Ãƒ ^ê%FWå£¨ë‹S~�ß»˜ï/v€\uI ¿^1Ù¯þæÝád•¡[A&Iç�D0{Èz¦>ùû�šæM„©—í£U¿5‰²¾¾�ïÕH¬œ ¼UöÔâäù‰Qô#øü›�«YáÅÓÖ)Ž¦¹ØM¯¬P·%Á:›:‡¾<¨Ítì
Ø¿â'pÈ.#Þ—xø¤ –!”�½èÄ5ÙŠÝp¤[îÉ,¾ÕGÁ|€#t�Ñ&éù{}oµ
)!g[.±wÂ#½ªLE)À×c¾Œ~EHª×µûÍhr\ñ ?\è�ÚíŸ’¦(8mˆ˜8²Iê^·s?‹½ê}œYñwX×$dãvËŸ‰�G¡_�¥}hÖîñc+÷ß”[ç«Ä0õ*³Á)ò_04Ð�ÖÌÑPARr};T˜IFìÓzùfgöJ»*	ŠR…BÚ¤Bð)bïw8»°ïò¹;ŠwÏTŸdF£º¥Ô=´u¡Ä“Žœ7úøjàÝ|lÃ´ i•ë´þ'ÿU³°vKC¿‰(ª9´]2ËÛeŠ‘b½{Vßdj�#ƒ”o…˜¾xæ‡ÖiÌvéÚ�D9"�Ä†âæx#ùr¬wÒÿÖ� �xÃ‰_ŠgrCSŸðŠ‡%à»8ûP‡zõ9Úv­à—þ?aYdnÝvë`	ö(ÎÑÆ¦aÝ–»	íVf™ú¾�:Ã ¹¤=„4=´¯¶ËWÛªŒÁ {<ûù A,¯ùMêÏßiÚ–	BÃ¸Kùõ—XâÁøŸ „[Ç§xQö;Ž{é§ŸlwZ£nKä"èœ3aŒ¥ãdÝ¬IÕ`Å¬kSE5ëˆF#ª‹ú�.±Ç/•z•ŸÛÎÒ �?Iuâ—aøÃü1d¿œÝ½*ú¥Ò"¯¯:1Ë6¥(<¶±¼ÝÎ¾±b$5U�Jsvô…í¨y¿ŒbQ9®&jn�	þ›Aßz}Èpî†�€íÙ5pLY.4•d %‹q›*a±‡Â#Bçƒî˜L®hÖ¹ìI·aC:z©rºg ü"²ýÌ»xÝ­5>8ç“_V«šqÑmŸqg(„àö¶ì=ÃÖTDÛS‡«0¹ž£,‡Bš¶�òyv1qtøŒ¥Ö5gïSM)�Œ ÛWª@î0z<û™�ßÛ>s~+÷JÀ`ÔÅ¬ÆîiøxÐoŽ|‚®Æ¦g´¶!3‚ônÁýä¦ðËié	-•Î½ýÉìgKk”%3Ì7‰ŸWë9ÚªEW‘*é#Í™©´X–@4gp±«÷›“òão¸µïJçb…jµÙŸ^ÌžGöjÿc
ÍM~!ÄÍ‹ü“nq~lô�¡¦BnðRžíª‹ÃTya¤v>w‘÷›AVxj\6a*
=ÂåôÝxÓï‹™/?Py%ÊmTG¿¡ú¸3ÅX8²¿¿z
g™-•.=«"_]µ'ÂÁ ow:f‹ae	=Òþô‹¿~ÿdeE]JÐ©Ç¯µåsãå¿p-îký%6ñ†©Á˜�ëúz²Bht$C@ H‰¯o=­T¸Nf{Õ>‰FU¬�¼¶HÊ4‘Â\ËHîÁpnØºÚ"iÓ\ùöÎÞë“—ô¾ÂÛÞº8S‰î(x?� �,äK¼7šÊ'þÝÅxHbƒ'‰£:1~Éh—VÈT�¶ìÏ;sï•öÊ¼YÜ[@a$Á¼JõLÏå}„ÇIÊÀ)ê„æç[‰¸+“ß‰;m•…æþ:ñ0$)í�ÓìEOL¼¿µ‚zvz>×ò»ûR‰áÒ\ÚòÍ–wËé�AXÊNƒýï”K¼æªÔ�ñ…ŽB÷€ï»Ñ«�%CQ*œ;~Qæ£WU@R‘bLnöý—]#‚�À2Aæ˜ë“�¢6x¥ñG�_a"ÝkÁêlµŠOVîºjrÉ�Ä¤& «.ò2­b”õdŒÚ%èKÁ4²ubx—¼"ú¿ž®‡õó¢ýä³ÞÍ¬dlA£
E5Å‘QlºÃP³ùÄ£¿nµ9géÜ°3Ü€?ô’ê0Ü¸,¼ŸÉ"(¢oø²…ŽàØ‹ô@…xdv¾¿¨Þ¼ÿæDI(�6•å ôay0÷{æ=r©sS÷Y“Ø+Ba#Ù|âlƒI£ÿ*Þˆžgš/ D)•Æ.këí	6$³öi›‚å¹xöât·zÐT£Q*l¥u0ÙÃäv±Í u/ì´46§.VòÉ4ý\ç(+^×Ç´ˆ¦â� @Ž%s-�w°_Pÿ�SâÖ,KÈR/}$ß hú\CÒ+óŸÖlYk™—EùXAP†êb4o3Pé>j0oäƒ‘<tZ�D[ßMÎÃa»&ÈQÒF!L‘«gFƒÖæÀ‰Ÿz#¥@u²%ÒîÜŠú‚ðÓ5ècCÞ£‚,¢$zÜöÝšƒXù>¼eÕEÈLêô7LP…Å¦:¶§ž_Æ¹ÅŸ?õ™6I�¸€p/ÓrëS%X3"S+ZÞ‡7Ùv „ÐáY*Þ´“|Ù÷ÖóÊ\yï�„º—V-çOˆˆ/ôÏUtâ~êÂC•¥U+/Üa„èV7²Pu—S¼H’?:ƒ:”˜Ø‚ÊM#	yO£Y>Ø§Åº'FlÎô'Qr5Ë¦ˆä’ŠÜÏŸÀÒø÷4‘Ze†œxó%ìilØŸTnANT6â�Ý¦éådmºP'j!‘||ª¬†À-,ŒÕ¥Pä÷Ef26ÇŠ—,ãOjÓ,
D›g¶!n"Ë= c¨]”îéÄ±ÇóŽs�^�3½(Ìÿ‰n µè«¾�RŠ„®Ý³ƒÄÞ>ÌøíªmâË¯!h\NÍL¦JÀ[©Y«Ž|£Ë[R)ZQ'UàÄD	†s‰ç×ôtWÐ‚#ÉpÓPOæñ¹]P¥dCÙ%²-ß–çÐ[ þh¯h
Û5Õœû¯¿Û8(ûˆ\Ò¿#Ä÷HŽxÃ%œ›-<0¬í*"GÄ–C]:W jÂpOa•OžÍBjˆâ ß�_¼½Üq­jÒÆ×'ŠžmƒüÕÌí•iLi­)¤’-#à0€ÀeãH…šm“âp¡;žh§Ã	ÜJåPâuHàŒ¤fz¯Ì\Y°­Ú÷W-Ü¾ù&	ƒaþ³ôN©mRÈò¢©�£Ó¸à4xÕW¯66µx½r«©ƒ@H(®n¤¹j(ïcÀ@)âläðøêšhmí àõÊôÈ¢ôRúÅÂçØ•<ëkæË¿4ž-c]Sý'±Œ6šbŸ)éª€1L${"
#õhZ³çp¯ŒBÞN\%-• ¥áºÎÞ¯ÝÑóŒ�QÇ‚§wRÒ±ÅWÝ]\ŸCvHÊ«›-&Ó¶àç9ÂI`j.áð¬÷íÒºo	ùÝ·¶ªé¼o|/>†Ù.óhþÕÇLyZL>º@U~BO%ò…2˜–ÁZ‹Jóó5�Þôí×ÇŠåÛ›ÖZ¨A%^¼þ©ïTImºè”Q™þfÔ ]x^#ì™K8“o:-1´÷à¹êRUÐT0ãÎÏŽU2Î™xºÙØZSO� (\\%>$Ž_ W‡M/´Ä3ÇçÃˆgAƒaÝºkì:Tå÷Ý5Š®^¸ËÏà$éåç]Ÿ�õÁg‚?'œÁL,˜c55L ®è¯³XÀ§�3{ƒL)ÍR�o.¼¢býcÛ‚7˜­?F)4Éî×&MÏöiÖ8ou@ucKuÛõ‰åvP7‹üDáõ«ö­+ÛV$÷[b„›ÀI•ò2—ŸÖøÎné÷xÿ0�6¢L"8Ôú©«…ú™
¦‡¡<éŽ¤¾!Ùñ>Åe£ðgÑ^*–C¶“Ô•HËA8VV$bLOÂ.ŽBRôúO£p.êŠ™p;™J—£AHGŒÃlïå·²°nýùõÝ*-Ñ›kñ°Ð¶–²´ÝˆïÊÝë¼‹-ö<•/Ç�¶LKèÖ„fH”6R+A‡à.TÕPO‚m
˜ÅÇ÷þ!ÔyÆäËOÇ£õ©#LæLÅ®b¹g9¾ÀÐ.1»uÕÐUû­]"¥»�bH°ŒÕóÊ	 pY‹U;†à1;Œ
BiÅ	0tÌ\1¯‰ak¿»†˜J«¿¶ÙRä^kQËÍ2áÍÚS	Â™µáÕ4Ñ¬/gš EÂÉ8Zý¿ü˜Zšh\ëµ»B‡’–ô·eí†CfCz[àœž~ûóñ«&(|83¨¦@kaÀëžfÅ}å¤˜œ¨Ç«„ê²yÖÍdßB�� ½å%‘ô_Y¶n{�æ¸­©w'ò�-FìÜƒK­ÈS‡”O¬µ{Ðï�¬IØloÙ€2 ¾^†àþ;ïÇd�~ Ô�¸Ñ;ÑH6këtÃ÷ƒ&ç6cçfÙàœE!–vÇ;ËcLvË1U¸aà&ØqGpX}P‚ón¶^›1}Ë�QÇ�ð�4/1‹êçûSXç4�Ù:(ù……S³s/@-Ó×Ô°eW¶Ñ¤ª[¸O˜\/%    ˆ"'¼R;l˜ «4¼§ ÿ¼?ñ±Ägû    YZ  |      g®ZTR

BASE      Q]  xÁA  ƒ°YëñÀ þµ,9•R›C¡ÑLfnÛ¶¤Zen«Ì%­¤Ze¬Ö&ÉÖ66kccc+Y±ªeÅ´˜ªÓL�VÕ6ÙlÛ Û2m)¹Z.ˆæXK’£lLbÁ˜šK˜(¸­`2,Ó )ÂRJb2$R“˜6SZL™L†*,—ªY$i3…I2$aQ0,tQn£r	VER…å²¹,S²Ü,Á¤L�é²ƒ¨Ü†@1	h1œš!n£P"�²i‚aPnn.,H†M T–"Qnn“¤X¦¡Ú"ÅMF2sŽ™i§6…M*“4Mær£˜“V™b…@ cÎ�2‹afÁ`ºTCÐÜ˜!P:KsS9	m�L±AÑšÉ m‰‹£Ög�€¨k›¶Ü6k´@]Û6\êV`{û@:ò  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>  g     R®ZTR

BASE      <  xÁA  ƒ°YãxÔ þµ,¹mk#ÛbS6q›ÌšnmMFK¶%³¦Tks9-§fªkmº—SRVŠ²Ñ6Qœnd¥9µ6Dik°-lÌÜB›l.´ŒXK4 9²áVbæX…©‘¬cà¢ÁX…©‘¬cà¢É°ÂÔHÖˆ1pl¬ÂÔHÖˆ1pÑPVaj$kÄ¸È�U˜É1."e¦F²FŒ�‹–c‹Íµpk›Ó`2f®btÉÈiJÄªeÖFºM�›NuÎ%Ñeh8£µÎfM«EÒ\�Ö*j2‹ ­˜“D �€IU%6)cµ¶j)`’Ð"2a A°"qÁb „Š\4V@ÀÀ’æ’ªaâÜrE¬’5´ÐIa8MÜÆØ1"  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>  t     _®ZTR

BASE      IA  xÁA  ƒ°YëñÀ þµ,9•T›C¡ÑLfnÛ¶¤Zen«Ì%­¤Ze¬Ö&ÉÖ66kccc+Y±ªeÅ´˜ªÓL�VÕ6ÙlÛ Û2m)¹Z.ˆæXK’£lLbÁ˜šK˜(¸­`2,Ó )ÂRJb2$R“˜6SZL™L†*,—ªY$i3…I2$aQ0,tQn£r	VER…å²¹,S²Ü,Á¤L�é²ƒ¨Ü†@1	h1œš!n£P"�²i‚aPnn.,H†M T–"Qnn“¤X¦¡Ú"ÅMF2sŽ™i§6…M*“4Mær£˜“V™b…@ cÎ�2‹afÁ`ºTCÐÜ˜!”ŽÁRsS9	m�L±AÑšÉ m‰‹Pk„³F@‹©knVn›5Zl¼ì3K  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>  w<AlignableSequences trimStringency="Medium"><Sequence name="13_G4221-F1_TSS20210513-027-03409_G09" ID="0" sortOrder="0" trimmedRange="15..1092"/><Sequence name="13_G4221-F2_TSS20210513-027-03409_A10" ID="1" sortOrder="1" trimmedRange="3..1030"/><Sequence name="14_G4221-F1_TSS20210513-027-03409_H09" ID="2" use="0" sortOrder="2" trimmedRange="15..1086"/></AlignableSequences>  §‹     ÿ BC N srôe”```pðpáó³2Ô3ãö·²*Í+Î/*IMábÊqqQjZjQj^r*C¥ gÛ.Ê6   ‹     ÿ BC ;í—¿ª%EÆ�ÿ,z«è Ón:h0Ye#AØ.
)ÃÍLÌ| »¨ BÅ'ÁÐÐ‡0_¿¹çâêYdAæƒžžSÓÓýus¨ßÔ/ï^ôù;—ËÝË/ø}Üùöå/zzù×Ÿ>»\ýWo]ûËcÿôîÚßã÷·h*©VÄYûž%š-"Š™5q—UvJîŒÍ-+I´JL¶™SJõš;Ò‹yQ-fº43©´²‰çdª¦;$êŽÚ}¯¶cJqNã2›·èMmE/CÔUoRZÇKÞ%çHUŸ1›°x(nt›ÎÚô6Tšû¨ªfóxäÚËÄàèuzi2–ç*ÙfbËœ’mH‹j¥ZÀÙñÔë¦)Ì Ø­{YgéÙÄ—s^Xeô9hÂý€›±ƒÒ*þ³G[º«Tóˆ“›ÄôcGªÛCVi‡Ã…K„µQtk'ÅvàA†i´X¥¨—‰�À×p™Ži½�ÖÖâÃ*,ÁOí%;Ã¶
·Ñ5¨™OøŒ…cÄO5Ž2‡1Ž]põ½MxR•åÁŠ½Töe{öY»®M‹­.*ÉÞ·­D¼B)wïôòÔ©S§þ}ýÈ¶gO®lû ÷ïÝ°íãË3\Ÿ?²ìþ£#r¹|ƒöíûûkü»Ë+Æý¯hª±ö5Õa•#³j˜”Šl
©·�w‚œšHŸ³wáRh"4@j�Y[/H†d¶©Y9•¹…-L>—¸n@@Çî9k	•jÁ ½ç(ù�kAÚ½rm¹�Há›ékoõÛÈäÛH}mŒ�Ø›±†Ž¾78Ç–%¨[[ Œ–7æL;ìà§}tÃk&O–Ç‘‚§»ÊL«-FÒ$À)kà<+…íF›„¶ÔÖ(ŸXÎÑr¼šá�ð%@±=	tb0³¢]²§e/‘ð`ïº{·MK`'vPÉ«&]³�a#+hO^‚çîRó�Žï›õüMÿÃO�:õÆõãMõá�”Ð|òPDÝzÈÏhtôw_Tý€VØHœEÕÿ°¨º’þUýOEUCQµˆ-ÿ(ªÎ2êÔ©SÿZ¿Ä/ßš  ‹     ÿ BC             !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  2´<?xml version="1.0"?><Features nextValidID="30"><Feature recentID="0" name="SP6 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2330-2348" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage SP6 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1365-1446" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="eBFP2" directionality="2" translationMW="26904.61" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1572-2291" color="#3366ff" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIKVNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLLEFRTAAGITLGMDELYK*"/></Q></Feature><Feature recentID="3" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3226-3228" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3229-3241" color="#ffcc99" type="standard"/></Feature><Feature recentID="5" name="spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="45-47" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3207-3225" color="#ff0000" type="standard"/></Feature><Feature recentID="7" name="STR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="918-936" color="#ff0000" type="standard"/></Feature><Feature recentID="8" name="Spacer" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="887-917" color="#a6acb3" type="standard"/></Feature><Feature recentID="9" name="ITR" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="874-886" color="#ffcc99" type="standard"/></Feature><Feature recentID="10" name="ChÎ²GI" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1115-1344" color="#a6acb3" type="standard"/></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-105" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="131-586" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4134-4722" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5684" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4893-5684" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5685-5753" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="15" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3780-3786" color="#ffffff" type="standard"/><Segment range="3787-3804" color="#ffffff" type="standard"/><Segment name="-35" range="3805-3810" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3825-3846" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="728-744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3732-3748" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3756-3772" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2814-2935" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="TRE3GV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2377-2723" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G; optimized for retroviral and lentiviral vectors&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2598-2616" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2562-2580" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2526-2544" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2490-2508" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2454-2472" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2418-2436" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="tet operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2382-2400" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;tetracycline repressor TetR&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;tetO&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bacterial operator O&lt;sub&gt;2&lt;/sub&gt; for the &lt;i&gt;tetR&lt;/i&gt; and &lt;i&gt;tetA&lt;/i&gt; genes&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2730-2761" color="#99ccff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;multiple cloning site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  §<Notes>
<UUID>48241487-bbab-4c88-a24a-163d757d4b36</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PiggyBac-TRE3GV-MCS-SP6-eBFP2</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.3.4</Created>
<LastModified UTC="12:10:41">2025.9.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y      K         
?????                                                                                                       ÿ d      DO  P              E              ÿÿÿÿYô? P                                                                                                                                                             a<StrandColors><TopStrand><ColorRange range="0..1343" colors="black"/><ColorRange range="1344..2389" colors="red"/><ColorRange range="2390..5752" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..1343" colors="black"/><ColorRange range="1344..2389" colors="red"/><ColorRange range="2390..5752" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
